Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16959 | 3' | -56.6 | NC_004322.1 | + | 271 | 0.66 | 0.148926 |
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Target: 5'- aCCCACuuGccauaaccccacACACCCACgccg-CCGCc -3' miRNA: 3'- gGGGUGggC------------UGUGGGUGguaaaGGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 868 | 0.66 | 0.148926 |
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Target: 5'- aCCUCACCCccgaacgcagGACGuuCACCA---CCGCc -3' miRNA: 3'- -GGGGUGGG----------CUGUggGUGGUaaaGGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 2282 | 0.66 | 0.133973 |
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Target: 5'- uCCUCACCgGAgaccccaggcagucCACCCACCAcgaaUCCa- -3' miRNA: 3'- -GGGGUGGgCU--------------GUGGGUGGUaa--AGGcg -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 2829 | 0.68 | 0.097687 |
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Target: 5'- gCCC-CCCGugACUCACUucc-CCGUg -3' miRNA: 3'- gGGGuGGGCugUGGGUGGuaaaGGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 4297 | 0.69 | 0.072502 |
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Target: 5'- gCCUACUCGcagaACACCCcaACCug-UCCGCc -3' miRNA: 3'- gGGGUGGGC----UGUGGG--UGGuaaAGGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 5509 | 0.71 | 0.05931 |
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Target: 5'- gCCCCAgCCCcACcCCCACCAUUacccUCGCc -3' miRNA: 3'- -GGGGU-GGGcUGuGGGUGGUAAa---GGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 5743 | 0.72 | 0.048456 |
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Target: 5'- gCCUCACCCGugAaCCCACCc---CCGa -3' miRNA: 3'- -GGGGUGGGCugU-GGGUGGuaaaGGCg -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 1364 | 0.76 | 0.020466 |
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Target: 5'- cCCCUGCCCGucaaugagguggacGCGCUCACCAccgCCGCu -3' miRNA: 3'- -GGGGUGGGC--------------UGUGGGUGGUaaaGGCG- -5' |
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| 16959 | 3' | -56.6 | NC_004322.1 | + | 1534 | 1.14 | 0.000013 |
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Target: 5'- gCCCCACCCGACACCCACCAUUUCCGCg -3' miRNA: 3'- -GGGGUGGGCUGUGGGUGGUAAAGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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