miRNA: 3'- -AAAAAUGACAAGCACAGCCAGCGUUA- -5'
miRNA: 3'- aAAAAUGACaaGCACAGCCAGCGuua -5'
miRNA: 3'- aAAAAUGaCAAGCACAGCca-GCGUua -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16970 | 3' | -46.8 | NC_004323.1 | + | 57513 | 1.05 | 0.02963 |
|
Target: 5'- aUUUUUACUGUUCGUGUCGGUCGCAAUg -3' miRNA: 3'- -AAAAAUGACAAGCACAGCCAGCGUUA- -5' |
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| 16970 | 3' | -46.8 | NC_004323.1 | + | 57317 | 0.77 | 0.814516 |
|
Target: 5'- gUUUUGCUGagCGUGUUGGUCGUc-- -3' miRNA: 3'- aAAAAUGACaaGCACAGCCAGCGuua -5' |
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| 16970 | 3' | -46.8 | NC_004323.1 | + | 9020 | 0.66 | 0.999658 |
|
Target: 5'- aUUUUGC-GUUCGUGUCGcugUGCAc- -3' miRNA: 3'- aAAAAUGaCAAGCACAGCca-GCGUua -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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