Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16971 | 3' | -52.7 | NC_004323.1 | + | 28854 | 0.73 | 0.719104 |
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Target: 5'- gCGACGcuucaauuGCGAaauaGCGCGCAGCcUGGCCa -3' miRNA: 3'- -GCUGC--------UGCUgc--UGCGCGUCGuAUUGG- -5' |
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| 16971 | 3' | -52.7 | NC_004323.1 | + | 47982 | 0.74 | 0.688588 |
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Target: 5'- uGAUGugGACaACGUGCGuGCAUgGACCa -3' miRNA: 3'- gCUGCugCUGcUGCGCGU-CGUA-UUGG- -5' |
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| 16971 | 3' | -52.7 | NC_004323.1 | + | 120027 | 0.76 | 0.595393 |
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Target: 5'- aGGCGACGGCGGCGaguugGCGGCAggcguUGGCa -3' miRNA: 3'- gCUGCUGCUGCUGCg----CGUCGU-----AUUGg -5' |
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| 16971 | 3' | -52.7 | NC_004323.1 | + | 67954 | 0.76 | 0.58507 |
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Target: 5'- gCGGCGACGACGACcauuGgGUAGUGgcGCCg -3' miRNA: 3'- -GCUGCUGCUGCUG----CgCGUCGUauUGG- -5' |
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| 16971 | 3' | -52.7 | NC_004323.1 | + | 120731 | 0.8 | 0.393527 |
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Target: 5'- cCGAUGACGACGGCGCGUGuuGUcACCa -3' miRNA: 3'- -GCUGCUGCUGCUGCGCGUcgUAuUGG- -5' |
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| 16971 | 3' | -52.7 | NC_004323.1 | + | 62114 | 1.14 | 0.003012 |
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Target: 5'- aCGACGACGACGACGCGCAGCAUAACCg -3' miRNA: 3'- -GCUGCUGCUGCUGCGCGUCGUAUUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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