miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16972 5' -59 NC_004323.1 + 106329 0.66 0.790538
Target:  5'- uGGUGUGUGCCGgCGGau-UGGUGCa -3'
miRNA:   3'- cCCAUGCACGGCgGCCgcuGCCAUGg -5'
16972 5' -59 NC_004323.1 + 66423 0.66 0.781636
Target:  5'- ----cCGUGCCGCCGuGCGACaacaaGUcuGCCg -3'
miRNA:   3'- cccauGCACGGCGGC-CGCUGc----CA--UGG- -5'
16972 5' -59 NC_004323.1 + 109451 0.66 0.768049
Target:  5'- uGGUACGcucaacaauuugcugGCCGCUaucgaaaacuugauaGGCGGCGGUGg- -3'
miRNA:   3'- cCCAUGCa--------------CGGCGG---------------CCGCUGCCAUgg -5'
16972 5' -59 NC_004323.1 + 44122 0.67 0.763462
Target:  5'- cGGGUAaaGUGCUGCgaucuuaauuaCGGCGGCGucccGCCg -3'
miRNA:   3'- -CCCAUg-CACGGCG-----------GCCGCUGCca--UGG- -5'
16972 5' -59 NC_004323.1 + 53837 0.68 0.687051
Target:  5'- cGGGcACG-GgCGCCGGCGGCuGUGg- -3'
miRNA:   3'- -CCCaUGCaCgGCGGCCGCUGcCAUgg -5'
16972 5' -59 NC_004323.1 + 64795 0.74 0.35579
Target:  5'- aGGUACc-GUCGCCGGCGGCacGUACCc -3'
miRNA:   3'- cCCAUGcaCGGCGGCCGCUGc-CAUGG- -5'
16972 5' -59 NC_004323.1 + 64952 1.13 0.000862
Target:  5'- cGGGUACGUGCCGCCGGCGACGGUACCu -3'
miRNA:   3'- -CCCAUGCACGGCGGCCGCUGCCAUGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.