miRNA: 3'- uuGCGACGGUaUCGCAGAG----UCAUGCG- -5'
miRNA: 3'- uUGCGACGGUaUCGcAGAGUc-AUGCG- -5'
miRNA: 3'- -UUGCGa-CGGUaUCGCAGaguCAUGCG- -5'
miRNA: 3'- -UUGCGACGGUAUCGCAGAGUCAUGCG- -5'
miRNA: 3'- -UUGCGACGguauCGCAGAG-------------UCaUGCG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16973 | 3' | -53.2 | NC_004323.1 | + | 21843 | 0.77 | 0.41745 |
|
Target: 5'- -uCGCUGCCA-GGCGUCUUcauuAGUAUGCc -3' miRNA: 3'- uuGCGACGGUaUCGCAGAG----UCAUGCG- -5' |
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| 16973 | 3' | -53.2 | NC_004323.1 | + | 33110 | 0.68 | 0.871179 |
|
Target: 5'- cGCGCUGCCAcAGC-UUUCAcaUACGCc -3' miRNA: 3'- uUGCGACGGUaUCGcAGAGUc-AUGCG- -5' |
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| 16973 | 3' | -53.2 | NC_004323.1 | + | 77604 | 0.72 | 0.682692 |
|
Target: 5'- aGGCGCcgGCCAcAGCGUCguuGUACGUu -3' miRNA: 3'- -UUGCGa-CGGUaUCGCAGaguCAUGCG- -5' |
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| 16973 | 3' | -53.2 | NC_004323.1 | + | 85994 | 1.1 | 0.003103 |
|
Target: 5'- cAACGCUGCCAUAGCGUCUCAGUACGCg -3' miRNA: 3'- -UUGCGACGGUAUCGCAGAGUCAUGCG- -5' |
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| 16973 | 3' | -53.2 | NC_004323.1 | + | 100050 | 0.68 | 0.892365 |
|
Target: 5'- uAACGCUGCagguGCGUCUCcgcguggauaucgAG-ACGCg -3' miRNA: 3'- -UUGCGACGguauCGCAGAG-------------UCaUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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