Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16973 | 5' | -63 | NC_004323.1 | + | 35308 | 0.66 | 0.574432 |
Target: 5'- cUGCccGAUC-CGCCGCCGCaCCAACa -3' miRNA: 3'- uGCGc-CUGGaGCGGCGGCGcGGUUGc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 10711 | 0.67 | 0.536048 |
Target: 5'- gACGgGGugUcgCGCaaCCGCGCCGACc -3' miRNA: 3'- -UGCgCCugGa-GCGgcGGCGCGGUUGc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 103541 | 0.67 | 0.51625 |
Target: 5'- uUGUGGGCgUCGCCauguacgGCCGCGgcuggaCCGGCGu -3' miRNA: 3'- uGCGCCUGgAGCGG-------CGGCGC------GGUUGC- -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 44430 | 0.67 | 0.498597 |
Target: 5'- uUGCGGAUC-CGCCGCCgguuucugauugGCGCgCAAUu -3' miRNA: 3'- uGCGCCUGGaGCGGCGG------------CGCG-GUUGc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 85796 | 0.69 | 0.427553 |
Target: 5'- uGCGCGGACgUUGgCGCgGCGgCGAgGu -3' miRNA: 3'- -UGCGCCUGgAGCgGCGgCGCgGUUgC- -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 117865 | 0.69 | 0.419096 |
Target: 5'- -gGCGGuAUCgCGCaCGCUGCGCCAuuGCGc -3' miRNA: 3'- ugCGCC-UGGaGCG-GCGGCGCGGU--UGC- -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 31932 | 0.69 | 0.386309 |
Target: 5'- aACGCGGGCCcacgUGCuggCGuuGCGCCAAa- -3' miRNA: 3'- -UGCGCCUGGa---GCG---GCggCGCGGUUgc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 16837 | 0.7 | 0.378381 |
Target: 5'- cACGCGuGACCgcuUCGUCGCCGCaGUCAcACu -3' miRNA: 3'- -UGCGC-CUGG---AGCGGCGGCG-CGGU-UGc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 126912 | 0.7 | 0.370563 |
Target: 5'- -gGCGGACggaUCGCUGCCGCGaCAAa- -3' miRNA: 3'- ugCGCCUGg--AGCGGCGGCGCgGUUgc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 85895 | 0.7 | 0.362856 |
Target: 5'- uGCcCGaGCCaaCGCCGCCGCGCCcGCGc -3' miRNA: 3'- -UGcGCcUGGa-GCGGCGGCGCGGuUGC- -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 107059 | 0.71 | 0.318985 |
Target: 5'- cCGCGGcCCUCGuuGUCGCGaaAACa -3' miRNA: 3'- uGCGCCuGGAGCggCGGCGCggUUGc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 73629 | 0.73 | 0.221337 |
Target: 5'- gGCGCaGGACCUgccacugCGCCGCCGC-CCAGa- -3' miRNA: 3'- -UGCG-CCUGGA-------GCGGCGGCGcGGUUgc -5' |
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16973 | 5' | -63 | NC_004323.1 | + | 85960 | 1.07 | 0.000884 |
Target: 5'- aACGCGGACCUCGCCGCCGCGCCAACGu -3' miRNA: 3'- -UGCGCCUGGAGCGGCGGCGCGGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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