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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16974 | 3' | -54.2 | NC_004323.1 | + | 20742 | 0.66 | 0.911697 |
Target: 5'- aAGGugAaGACcaGCAGCugGUGCCGu-- -3' miRNA: 3'- -UCCugU-CUGa-CGUUGugCACGGUcuu -5' |
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16974 | 3' | -54.2 | NC_004323.1 | + | 64594 | 0.69 | 0.801526 |
Target: 5'- uGGGACAGA--GCAACGCG-GCCGa-- -3' miRNA: 3'- -UCCUGUCUgaCGUUGUGCaCGGUcuu -5' |
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16974 | 3' | -54.2 | NC_004323.1 | + | 90803 | 1.06 | 0.004225 |
Target: 5'- cAGGACAGACUGCAACACGUGCCAGAAg -3' miRNA: 3'- -UCCUGUCUGACGUUGUGCACGGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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