Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16974 | 5' | -48.2 | NC_004323.1 | + | 78526 | 0.66 | 0.999225 |
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Target: 5'- gCGCUuuGAag-UCGACaAUAugAUUGUg -3' miRNA: 3'- -GCGAggCUguaAGCUG-UGUugUAACA- -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 10768 | 0.66 | 0.99883 |
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Target: 5'- uGCaUCCGAUAUUUGACAaCAACc---- -3' miRNA: 3'- gCG-AGGCUGUAAGCUGU-GUUGuaaca -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 115098 | 0.69 | 0.988457 |
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Target: 5'- uGCUCCuguuuUUCGACGCuuCGUUGUa -3' miRNA: 3'- gCGAGGcugu-AAGCUGUGuuGUAACA- -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 90635 | 0.69 | 0.986853 |
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Target: 5'- gCGCUCCGGCGcUUCuGGCACGuguuGCAgucUGUc -3' miRNA: 3'- -GCGAGGCUGU-AAG-CUGUGU----UGUa--ACA- -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 83539 | 0.7 | 0.981007 |
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Target: 5'- aCGCUCCGAUuuaauucaaaAUUC-ACACAACGcuaUUGUa -3' miRNA: 3'- -GCGAGGCUG----------UAAGcUGUGUUGU---AACA- -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 23308 | 0.73 | 0.927159 |
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Target: 5'- aGCUCCGucCAaUCGACGCGAUuuUUGUa -3' miRNA: 3'- gCGAGGCu-GUaAGCUGUGUUGu-AACA- -5' |
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| 16974 | 5' | -48.2 | NC_004323.1 | + | 90768 | 1.08 | 0.016989 |
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Target: 5'- gCGCUCCGACAUUCGACACAACAUUGUc -3' miRNA: 3'- -GCGAGGCUGUAAGCUGUGUUGUAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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