Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16977 | 3' | -51.3 | NC_004323.1 | + | 118294 | 0.66 | 0.988075 |
Target: 5'- gGCCGacugaacuUGGGUuucggCGCCuucacgCAugGCCGCc -3' miRNA: 3'- -CGGU--------ACUCA-----GCGGuuua--GUugCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 42453 | 0.66 | 0.988075 |
Target: 5'- -gCAUu-GUCGCCAA----ACGCCGCu -3' miRNA: 3'- cgGUAcuCAGCGGUUuaguUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 21870 | 0.67 | 0.980662 |
Target: 5'- uGUCucgaa-CGCCAAcgCGACGCCGUg -3' miRNA: 3'- -CGGuacucaGCGGUUuaGUUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 12392 | 0.67 | 0.978362 |
Target: 5'- aCUAUGAcUCGCgAAccaAACGCCGCc -3' miRNA: 3'- cGGUACUcAGCGgUUuagUUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 64324 | 0.67 | 0.978362 |
Target: 5'- gGCCAaagcGUCuuuGCCuaacauAUCGGCGCCGCc -3' miRNA: 3'- -CGGUacu-CAG---CGGuu----UAGUUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 106406 | 0.67 | 0.975866 |
Target: 5'- uUCAUGAGaUUGCCAAucaAACGCuCGUc -3' miRNA: 3'- cGGUACUC-AGCGGUUuagUUGCG-GCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 74749 | 0.67 | 0.975866 |
Target: 5'- cCCGUGGGUauggCGCCGcgacaaaugagAGUUAAcCGCUGCa -3' miRNA: 3'- cGGUACUCA----GCGGU-----------UUAGUU-GCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 100796 | 0.67 | 0.974811 |
Target: 5'- cGCCAUGAaacugauuauccUGCUGuuuGUCGugGCCGCg -3' miRNA: 3'- -CGGUACUca----------GCGGUu--UAGUugCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 106191 | 0.67 | 0.973166 |
Target: 5'- uGCCGauGGUCGggauCCAAAacUCGACGUCGUc -3' miRNA: 3'- -CGGUacUCAGC----GGUUU--AGUUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 77702 | 0.67 | 0.970253 |
Target: 5'- uGCCAUGAuacCGCUA-----ACGCCGCu -3' miRNA: 3'- -CGGUACUca-GCGGUuuaguUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 53807 | 0.68 | 0.963762 |
Target: 5'- cGCCGcaacAGcCGCCAGaAUCgAACGUCGCg -3' miRNA: 3'- -CGGUac--UCaGCGGUU-UAG-UUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 32436 | 0.68 | 0.956341 |
Target: 5'- cCCAUGucGUCGgCAAG-C-ACGCCGCc -3' miRNA: 3'- cGGUACu-CAGCgGUUUaGuUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 106608 | 0.69 | 0.947948 |
Target: 5'- cGUUGUG-G-CGCCAAGUgGGCGgCCGCu -3' miRNA: 3'- -CGGUACuCaGCGGUUUAgUUGC-GGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 109651 | 0.69 | 0.928131 |
Target: 5'- uGCCAUGAcGUUGCgAGA---GCGCgGCg -3' miRNA: 3'- -CGGUACU-CAGCGgUUUaguUGCGgCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 116297 | 0.7 | 0.890718 |
Target: 5'- aUguUGGGUCGCCAuuuuUCAacacggACGCCGUu -3' miRNA: 3'- cGguACUCAGCGGUuu--AGU------UGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 113989 | 0.72 | 0.844551 |
Target: 5'- cGUCAaGAGUCGUUAuuu--GCGCCGCa -3' miRNA: 3'- -CGGUaCUCAGCGGUuuaguUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 106051 | 0.75 | 0.678799 |
Target: 5'- aGCCAUGGaagugauGUCGCuCAAAcuggugcCGACGCCGCc -3' miRNA: 3'- -CGGUACU-------CAGCG-GUUUa------GUUGCGGCG- -5' |
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16977 | 3' | -51.3 | NC_004323.1 | + | 129705 | 1.14 | 0.003515 |
Target: 5'- aGCCAUGAGUCGCCAAAUCAACGCCGCc -3' miRNA: 3'- -CGGUACUCAGCGGUUUAGUUGCGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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