Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16977 | 5' | -58 | NC_004323.1 | + | 53811 | 0.66 | 0.779458 |
Target: 5'- gCAaCAGCCGCCAgaaucgaacgUCGCGGGCgucggcggCUCg -3' miRNA: 3'- -GUcGUCGGCGGU----------GGUGUCCGaua-----GAG- -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 62099 | 0.66 | 0.779458 |
Target: 5'- aGGCAcGCCGCUAUugUAUAGGUUGcugCUCa -3' miRNA: 3'- gUCGU-CGGCGGUG--GUGUCCGAUa--GAG- -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 1056 | 0.66 | 0.750669 |
Target: 5'- cCAGCGGCgUGCCGCaugcaacgCACAGG-UAUCUg -3' miRNA: 3'- -GUCGUCG-GCGGUG--------GUGUCCgAUAGAg -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 3892 | 0.67 | 0.710827 |
Target: 5'- gGGCAGCCGCCGCCcuucuuagcCAGaCUAauUCUg -3' miRNA: 3'- gUCGUCGGCGGUGGu--------GUCcGAU--AGAg -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 50205 | 0.68 | 0.607689 |
Target: 5'- gGGCGGCCGCUuCUACAGcGCaauacaUGUUUCa -3' miRNA: 3'- gUCGUCGGCGGuGGUGUC-CG------AUAGAG- -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 13305 | 0.68 | 0.607689 |
Target: 5'- aAGCGGCC-CCGCUuuGGGUUGUCg- -3' miRNA: 3'- gUCGUCGGcGGUGGugUCCGAUAGag -5' |
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16977 | 5' | -58 | NC_004323.1 | + | 129670 | 1.08 | 0.001342 |
Target: 5'- gCAGCAGCCGCCACCACAGGCUAUCUCu -3' miRNA: 3'- -GUCGUCGGCGGUGGUGUCCGAUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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