miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16977 5' -58 NC_004323.1 + 53811 0.66 0.779458
Target:  5'- gCAaCAGCCGCCAgaaucgaacgUCGCGGGCgucggcggCUCg -3'
miRNA:   3'- -GUcGUCGGCGGU----------GGUGUCCGaua-----GAG- -5'
16977 5' -58 NC_004323.1 + 62099 0.66 0.779458
Target:  5'- aGGCAcGCCGCUAUugUAUAGGUUGcugCUCa -3'
miRNA:   3'- gUCGU-CGGCGGUG--GUGUCCGAUa--GAG- -5'
16977 5' -58 NC_004323.1 + 1056 0.66 0.750669
Target:  5'- cCAGCGGCgUGCCGCaugcaacgCACAGG-UAUCUg -3'
miRNA:   3'- -GUCGUCG-GCGGUG--------GUGUCCgAUAGAg -5'
16977 5' -58 NC_004323.1 + 3892 0.67 0.710827
Target:  5'- gGGCAGCCGCCGCCcuucuuagcCAGaCUAauUCUg -3'
miRNA:   3'- gUCGUCGGCGGUGGu--------GUCcGAU--AGAg -5'
16977 5' -58 NC_004323.1 + 50205 0.68 0.607689
Target:  5'- gGGCGGCCGCUuCUACAGcGCaauacaUGUUUCa -3'
miRNA:   3'- gUCGUCGGCGGuGGUGUC-CG------AUAGAG- -5'
16977 5' -58 NC_004323.1 + 13305 0.68 0.607689
Target:  5'- aAGCGGCC-CCGCUuuGGGUUGUCg- -3'
miRNA:   3'- gUCGUCGGcGGUGGugUCCGAUAGag -5'
16977 5' -58 NC_004323.1 + 129670 1.08 0.001342
Target:  5'- gCAGCAGCCGCCACCACAGGCUAUCUCu -3'
miRNA:   3'- -GUCGUCGGCGGUGGUGUCCGAUAGAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.