Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16978 | 3' | -46.4 | NC_004323.1 | + | 113992 | 0.69 | 0.998415 |
Target: 5'- -aGACGuCAAGaGUCGUUAUUUgcGCCg -3' miRNA: 3'- ugCUGC-GUUUgCAGCAGUAAGauUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 117141 | 0.75 | 0.93457 |
Target: 5'- aACGugGCGu-CGUCGUCcgUUUcGACCa -3' miRNA: 3'- -UGCugCGUuuGCAGCAGuaAGA-UUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 119318 | 0.67 | 0.999829 |
Target: 5'- gACGugGUAGAUGaCGUCAacuguuuuauacUUCaagAACCa -3' miRNA: 3'- -UGCugCGUUUGCaGCAGU------------AAGa--UUGG- -5' |
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16978 | 3' | -46.4 | NC_004323.1 | + | 120739 | 0.66 | 0.999957 |
Target: 5'- gACGGCGCG--UGUUGUCAccauuuauUUUUGACg -3' miRNA: 3'- -UGCUGCGUuuGCAGCAGU--------AAGAUUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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