Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16978 | 5' | -49.3 | NC_004323.1 | + | 14901 | 0.66 | 0.998201 |
Target: 5'- uGAuuGCUGAAcgGAGacgauggguguaaaGACGGCgAUGGCu -3' miRNA: 3'- gCU--CGAUUUuaCUUg-------------CUGCCG-UGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 5428 | 0.66 | 0.99724 |
Target: 5'- --uGCUuacauUGAGCGGuuguuaaauauCGGCACGGUa -3' miRNA: 3'- gcuCGAuuuu-ACUUGCU-----------GCCGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 74760 | 0.67 | 0.995475 |
Target: 5'- -uGGCgcuAGUGccCGugGGUAUGGCg -3' miRNA: 3'- gcUCGauuUUACuuGCugCCGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 3738 | 0.67 | 0.991793 |
Target: 5'- -uGGCUAAGAaGGGCGGCGGCu--GCc -3' miRNA: 3'- gcUCGAUUUUaCUUGCUGCCGugcCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 7620 | 0.68 | 0.990576 |
Target: 5'- --uGCUAAauacAAUGAggugcACGACGuGUAUGGCg -3' miRNA: 3'- gcuCGAUU----UUACU-----UGCUGC-CGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 4744 | 0.68 | 0.98772 |
Target: 5'- gGGGC-AGGcgGGGCuGGCGGCGgaGGCg -3' miRNA: 3'- gCUCGaUUUuaCUUG-CUGCCGUg-CCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 46250 | 0.68 | 0.986061 |
Target: 5'- -aAGUaucaUAAAAUGAACGGCGGCgACGa- -3' miRNA: 3'- gcUCG----AUUUUACUUGCUGCCG-UGCcg -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 9877 | 0.69 | 0.9816 |
Target: 5'- aCGuGCacgucaaaccguUGGAUGACGGCGgCGGCa -3' miRNA: 3'- -GCuCGauuuu-------ACUUGCUGCCGU-GCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 129447 | 0.69 | 0.98005 |
Target: 5'- -uGGCUGcGAcugcuUGAA-GACGGCugGGCu -3' miRNA: 3'- gcUCGAUuUU-----ACUUgCUGCCGugCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 62310 | 0.69 | 0.975086 |
Target: 5'- uCGGGCgucGAGAUgGAACGGCcGCGCaGCa -3' miRNA: 3'- -GCUCGa--UUUUA-CUUGCUGcCGUGcCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 124984 | 0.69 | 0.975086 |
Target: 5'- -uAGCUGuuGUcGAACGAcgcuCGGgACGGCg -3' miRNA: 3'- gcUCGAUuuUA-CUUGCU----GCCgUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 88470 | 0.71 | 0.936512 |
Target: 5'- -cGGUgu-AAUGuuCGGCGGUACGGCg -3' miRNA: 3'- gcUCGauuUUACuuGCUGCCGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 85792 | 0.72 | 0.931267 |
Target: 5'- -aGGCUGcgc-GGACGuuGGCGCGGCg -3' miRNA: 3'- gcUCGAUuuuaCUUGCugCCGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 73797 | 0.73 | 0.894286 |
Target: 5'- --cGCUAcucUGGGCGGCGGCGCaguGGCa -3' miRNA: 3'- gcuCGAUuuuACUUGCUGCCGUG---CCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 16493 | 0.76 | 0.775238 |
Target: 5'- uCGcAGCUGGGAUGAguuguuggAgGACGGCACuaGGCg -3' miRNA: 3'- -GC-UCGAUUUUACU--------UgCUGCCGUG--CCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 36022 | 0.83 | 0.379788 |
Target: 5'- -aAGCgccccuGUGGACGAUGGCGCGGCg -3' miRNA: 3'- gcUCGauuu--UACUUGCUGCCGUGCCG- -5' |
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16978 | 5' | -49.3 | NC_004323.1 | + | 21140 | 1.13 | 0.006885 |
Target: 5'- aCGAGCUAAAAUGAACGACGGCACGGCc -3' miRNA: 3'- -GCUCGAUUUUACUUGCUGCCGUGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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