Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16979 | 3' | -49.3 | NC_004323.1 | + | 63444 | 0.66 | 0.997571 |
Target: 5'- gGCGCUCAacGACGAUAUcg---CCAGCGa -3' miRNA: 3'- -UGCGAGU--UUGCUGUGcuacaGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 22410 | 0.66 | 0.997108 |
Target: 5'- uACGCUCAAA-GACACGGcua-CGACGa -3' miRNA: 3'- -UGCGAGUUUgCUGUGCUacagGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 82322 | 0.66 | 0.997057 |
Target: 5'- -aGCUCAAacauuagacugccACGACugGGUGUUggaaAGCAa -3' miRNA: 3'- ugCGAGUU-------------UGCUGugCUACAGg---UUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 65635 | 0.67 | 0.994466 |
Target: 5'- uGCGUUUggGCGACGgCGuGUGUaCAGCAg -3' miRNA: 3'- -UGCGAGuuUGCUGU-GC-UACAgGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 117336 | 0.67 | 0.994466 |
Target: 5'- aGCGUcaauaGAAUGAgCACGAUG-CCGACAa -3' miRNA: 3'- -UGCGag---UUUGCU-GUGCUACaGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 64093 | 0.67 | 0.994466 |
Target: 5'- -aGCU--GGCGACGCGAg--CCGGCAa -3' miRNA: 3'- ugCGAguUUGCUGUGCUacaGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 85649 | 0.67 | 0.990148 |
Target: 5'- -gGUUCAAACgGACACGcUGUUgGACGc -3' miRNA: 3'- ugCGAGUUUG-CUGUGCuACAGgUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 10241 | 0.68 | 0.988737 |
Target: 5'- cACGgaaaUCAAugcCGACAUGucUGUCCAGCAa -3' miRNA: 3'- -UGCg---AGUUu--GCUGUGCu-ACAGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 73914 | 0.69 | 0.968029 |
Target: 5'- aACGCUUAGugGAUACGAaGaauacCCGACGu -3' miRNA: 3'- -UGCGAGUUugCUGUGCUaCa----GGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 28557 | 0.7 | 0.96108 |
Target: 5'- cACGCUCAAGC-ACACGAUGaaC-ACAg -3' miRNA: 3'- -UGCGAGUUUGcUGUGCUACagGuUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 97057 | 0.7 | 0.95724 |
Target: 5'- cCGCUCGAACauucGCACGc-GUCCGGCGa -3' miRNA: 3'- uGCGAGUUUGc---UGUGCuaCAGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 107806 | 0.7 | 0.95315 |
Target: 5'- gUGUUCAAugcCGACugGGUGUCCGu-- -3' miRNA: 3'- uGCGAGUUu--GCUGugCUACAGGUugu -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 47157 | 0.72 | 0.904535 |
Target: 5'- uCGCUCAu-CGACuuGAuauUGUCCGACAc -3' miRNA: 3'- uGCGAGUuuGCUGugCU---ACAGGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 74546 | 0.76 | 0.749296 |
Target: 5'- cACGCgUCGAACGACACGAUGgacgcgaaaaUgCAGCGg -3' miRNA: 3'- -UGCG-AGUUUGCUGUGCUAC----------AgGUUGU- -5' |
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16979 | 3' | -49.3 | NC_004323.1 | + | 28356 | 1.09 | 0.011391 |
Target: 5'- uACGCUCAAACGACACGAUGUCCAACAu -3' miRNA: 3'- -UGCGAGUUUGCUGUGCUACAGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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