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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16980 | 5' | -52.5 | NC_004323.1 | + | 57781 | 0.71 | 0.811462 |
Target: 5'- gCGAUcu-GGugUUGGCAGAugGCUCg -3' miRNA: 3'- -GCUGuacCCugAGCCGUUUugCGAGa -5' |
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16980 | 5' | -52.5 | NC_004323.1 | + | 64431 | 0.79 | 0.392745 |
Target: 5'- uCGACA-GGGACUCGGCcgcguUGCUCUg -3' miRNA: 3'- -GCUGUaCCCUGAGCCGuuuu-GCGAGA- -5' |
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16980 | 5' | -52.5 | NC_004323.1 | + | 32296 | 1.08 | 0.005942 |
Target: 5'- aCGACAUGGGACUCGGCAAAACGCUCUc -3' miRNA: 3'- -GCUGUACCCUGAGCCGUUUUGCGAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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