Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16982 | 5' | -56.9 | NC_004323.1 | + | 35471 | 0.66 | 0.856463 |
Target: 5'- -gGGCGcuuGUUGGugCgGCgGCGgAUCg -3' miRNA: 3'- cgCCGUu--CAACCugGgUGgCGCgUAG- -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 107362 | 0.66 | 0.84046 |
Target: 5'- gGCGGCucaUUGGAuCCCAacaCaCGCGUCg -3' miRNA: 3'- -CGCCGuucAACCU-GGGUg--GcGCGUAG- -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 126874 | 0.68 | 0.758927 |
Target: 5'- aGCGGCAAGUUaauGCCCAUCauuuuguugauagGCGCAg- -3' miRNA: 3'- -CGCCGUUCAAcc-UGGGUGG-------------CGCGUag -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 38906 | 0.71 | 0.577641 |
Target: 5'- gGCGuGCGGGUUGucauauuUCCGCCGCGCAc- -3' miRNA: 3'- -CGC-CGUUCAACcu-----GGGUGGCGCGUag -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 85813 | 0.72 | 0.494029 |
Target: 5'- gGCGGCGAGguccgcguuCCCGCCGCGUAc- -3' miRNA: 3'- -CGCCGUUCaaccu----GGGUGGCGCGUag -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 85970 | 0.72 | 0.488254 |
Target: 5'- cGCGGCGGGaaacgcGGACCUcgccGCCGCGCcaacGUCc -3' miRNA: 3'- -CGCCGUUCaa----CCUGGG----UGGCGCG----UAG- -5' |
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16982 | 5' | -56.9 | NC_004323.1 | + | 38700 | 1.1 | 0.001628 |
Target: 5'- uGCGGCAAGUUGGACCCACCGCGCAUCc -3' miRNA: 3'- -CGCCGUUCAACCUGGGUGGCGCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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