miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16983 5' -54.1 NC_004323.1 + 1038 0.67 0.88951
Target:  5'- ---cGAGCGGgUGGuCGA--UGACCaGCg -3'
miRNA:   3'- uuuaCUCGCCgGCC-GCUuaACUGG-CG- -5'
16983 5' -54.1 NC_004323.1 + 13082 0.66 0.926922
Target:  5'- uAAUGAGUuaaauaGGCgGGC-AGUUGuCCGCu -3'
miRNA:   3'- uUUACUCG------CCGgCCGcUUAACuGGCG- -5'
16983 5' -54.1 NC_004323.1 + 63866 0.97 0.020971
Target:  5'- cAAAUGA-CGGCCGGCGAAUUGACCGCg -3'
miRNA:   3'- -UUUACUcGCCGGCCGCUUAACUGGCG- -5'
16983 5' -54.1 NC_004323.1 + 106205 0.67 0.887406
Target:  5'- --uUGAGCgacaucacuuccauGGCUgaaGGCGAAUUaGCCGCu -3'
miRNA:   3'- uuuACUCG--------------CCGG---CCGCUUAAcUGGCG- -5'
16983 5' -54.1 NC_004323.1 + 120030 0.69 0.790216
Target:  5'- ---cGA-CGG-CGGCGAGUUGGCgGCa -3'
miRNA:   3'- uuuaCUcGCCgGCCGCUUAACUGgCG- -5'
16983 5' -54.1 NC_004323.1 + 126894 0.66 0.937376
Target:  5'- --cUGAGgcgaaucugGGUCGGCGGAcgGAUCGCu -3'
miRNA:   3'- uuuACUCg--------CCGGCCGCUUaaCUGGCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.