Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16984 | 5' | -50 | NC_004323.1 | + | 57014 | 0.66 | 0.995962 |
Target: 5'- cGAuGGCaaauacugaaugGCGACGAAAUCgaagugUCGCCAGa -3' miRNA: 3'- aCU-CUG------------UGCUGUUUUAGa-----GGCGGUCg -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 7915 | 0.66 | 0.994476 |
Target: 5'- uUGAGACAaGACGAAuUCUauGCCAa- -3' miRNA: 3'- -ACUCUGUgCUGUUUuAGAggCGGUcg -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 36037 | 0.66 | 0.994476 |
Target: 5'- cGAuGGCGCGGCGcauuugGAAUCgCCG-CGGCa -3' miRNA: 3'- aCU-CUGUGCUGU------UUUAGaGGCgGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 123958 | 0.66 | 0.994476 |
Target: 5'- aUGAuuGCACGGCGuauuAUCUCUGUCcGCa -3' miRNA: 3'- -ACUc-UGUGCUGUuu--UAGAGGCGGuCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 124019 | 0.66 | 0.994476 |
Target: 5'- --cGAauuUGACuuGGaCUCCGCCAGCu -3' miRNA: 3'- acuCUgu-GCUGuuUUaGAGGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 130803 | 0.66 | 0.993582 |
Target: 5'- --cGugACGACA----CUgCGCCAGCc -3' miRNA: 3'- acuCugUGCUGUuuuaGAgGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 64588 | 0.66 | 0.993582 |
Target: 5'- aGAGcaACGCGGCcGAGUCcCUGUCgAGCg -3' miRNA: 3'- aCUC--UGUGCUGuUUUAGaGGCGG-UCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 64187 | 0.67 | 0.99145 |
Target: 5'- aGAGugGCGuguuugagcaACAGAGUCauguaauugUUgGCCAGCu -3' miRNA: 3'- aCUCugUGC----------UGUUUUAG---------AGgCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 104200 | 0.67 | 0.985533 |
Target: 5'- aGGGACGCGAagauuuCAAAGUUgccauucacgaUCCGUgGGCc -3' miRNA: 3'- aCUCUGUGCU------GUUUUAG-----------AGGCGgUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 16725 | 0.68 | 0.974252 |
Target: 5'- gGGGACAgGACcucuAUgUgUGCCAGCa -3' miRNA: 3'- aCUCUGUgCUGuuu-UAgAgGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 130134 | 0.69 | 0.968299 |
Target: 5'- aGAGGaaaACGGCAGcGAUCUgCGCUccAGCa -3' miRNA: 3'- aCUCUg--UGCUGUU-UUAGAgGCGG--UCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 46899 | 0.7 | 0.944741 |
Target: 5'- gUGAGACGCGACGAAAaaagUCGUguGUu -3' miRNA: 3'- -ACUCUGUGCUGUUUUaga-GGCGguCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 122580 | 0.7 | 0.934855 |
Target: 5'- -cGGACcgGCGGCGAA--CUCUGCCGGUa -3' miRNA: 3'- acUCUG--UGCUGUUUuaGAGGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 97376 | 0.72 | 0.89197 |
Target: 5'- -cAGACugGGCuuGGuucacguuuUCUCCGCUGGCg -3' miRNA: 3'- acUCUGugCUGuuUU---------AGAGGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 113543 | 0.77 | 0.634702 |
Target: 5'- cGAGGug-GACAAGcugaagcGUCUCCGCCAGCg -3' miRNA: 3'- aCUCUgugCUGUUU-------UAGAGGCGGUCG- -5' |
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16984 | 5' | -50 | NC_004323.1 | + | 97191 | 1.14 | 0.004627 |
Target: 5'- uUGAGACACGACAAAAUCUCCGCCAGCg -3' miRNA: 3'- -ACUCUGUGCUGUUUUAGAGGCGGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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