Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16985 | 5' | -46.6 | NC_004323.1 | + | 29348 | 0.66 | 0.999863 |
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Target: 5'- aCUUUUUUUAacGCAGCGACAauuGUUUGcauaauauuGCCg -3' miRNA: 3'- -GAAAAGAAU--CGUCGUUGU---CAAGC---------CGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 96589 | 0.66 | 0.999822 |
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Target: 5'- ---gUCUUccagaAGCGuGCu-CAGUUCGGUCg -3' miRNA: 3'- gaaaAGAA-----UCGU-CGuuGUCAAGCCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 131067 | 0.66 | 0.999822 |
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Target: 5'- ------cUAGCAGCAGaAGUUcCGGCa -3' miRNA: 3'- gaaaagaAUCGUCGUUgUCAA-GCCGg -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 128617 | 0.67 | 0.999524 |
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Target: 5'- ---gUCUUGGCucGUAGC---UCGGCCa -3' miRNA: 3'- gaaaAGAAUCGu-CGUUGucaAGCCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 81713 | 0.67 | 0.999255 |
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Target: 5'- aCUUUgcac-GUAGCcACAGUgccgUCGGCCa -3' miRNA: 3'- -GAAAagaauCGUCGuUGUCA----AGCCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 88303 | 0.68 | 0.998308 |
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Target: 5'- -cUUUCcaaaagAGCGuuuGCGGCAGUuuguUCGGCCc -3' miRNA: 3'- gaAAAGaa----UCGU---CGUUGUCA----AGCCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 68220 | 0.7 | 0.989717 |
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Target: 5'- -----aUUAGCGGCGACGGUaauUCuGGCUc -3' miRNA: 3'- gaaaagAAUCGUCGUUGUCA---AG-CCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 107911 | 0.71 | 0.98655 |
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Target: 5'- ---aUUUUGGCAGCGGCGGUUa-GCUc -3' miRNA: 3'- gaaaAGAAUCGUCGUUGUCAAgcCGG- -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 65569 | 0.71 | 0.98471 |
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Target: 5'- --cUUCUugcucaucaaUAGCGGCGACGGaggCGGCg -3' miRNA: 3'- gaaAAGA----------AUCGUCGUUGUCaa-GCCGg -5' |
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| 16985 | 5' | -46.6 | NC_004323.1 | + | 107788 | 1.15 | 0.008242 |
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Target: 5'- cCUUUUCUUAGCAGCAACAGUUCGGCCg -3' miRNA: 3'- -GAAAAGAAUCGUCGUUGUCAAGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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