miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16986 3' -58.3 NC_004323.1 + 118310 1.1 0.001166
Target:  5'- gUUUCGGCGCCUUCACGCAUGGCCGCCg -3'
miRNA:   3'- -AAAGCCGCGGAAGUGCGUACCGGCGG- -5'
16986 3' -58.3 NC_004323.1 + 64348 0.66 0.742791
Target:  5'- -aUCGGCGCCgccguuguaaaacuUUUGCGCGUacGCCGgCa -3'
miRNA:   3'- aaAGCCGCGG--------------AAGUGCGUAc-CGGCgG- -5'
16986 3' -58.3 NC_004323.1 + 109807 0.67 0.675694
Target:  5'- --aCGccGCGCUcUCGCaacgucaugGCAUGGCUGCCg -3'
miRNA:   3'- aaaGC--CGCGGaAGUG---------CGUACCGGCGG- -5'
16986 3' -58.3 NC_004323.1 + 103539 0.67 0.7061
Target:  5'- -gUgGGCGUCgcCAUGUAcGGCCGCg -3'
miRNA:   3'- aaAgCCGCGGaaGUGCGUaCCGGCGg -5'
16986 3' -58.3 NC_004323.1 + 57080 0.66 0.745714
Target:  5'- --gCGGUgaGCCgcuuauaaaACGCAUGGCCaugGCCa -3'
miRNA:   3'- aaaGCCG--CGGaag------UGCGUACCGG---CGG- -5'
16986 3' -58.3 NC_004323.1 + 64489 0.66 0.764956
Target:  5'- --gCGGCGCCgauaugUUaggcaaagACGCuuuggccgaGGCCGCCa -3'
miRNA:   3'- aaaGCCGCGGa-----AG--------UGCGua-------CCGGCGG- -5'
16986 3' -58.3 NC_004323.1 + 34558 0.66 0.764956
Target:  5'- -gUCGuCGUCgUCACGCGcucGCCGCCu -3'
miRNA:   3'- aaAGCcGCGGaAGUGCGUac-CGGCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.