miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16987 3' -54.2 NC_004323.1 + 57875 0.66 0.943515
Target:  5'- gCUAAuUCUGUUUGUCUGcgucCCGGCGGu -3'
miRNA:   3'- aGGUUuGGACGAGCAGAU----GGCCGUCu -5'
16987 3' -54.2 NC_004323.1 + 97378 0.66 0.923016
Target:  5'- cCCAGACUggGCUUGguucacguuuUCUccGCUGGCGGAg -3'
miRNA:   3'- aGGUUUGGa-CGAGC----------AGA--UGGCCGUCU- -5'
16987 3' -54.2 NC_004323.1 + 236 0.66 0.923016
Target:  5'- uUUUAAACCUGaaCUCGcUUUACgGGUAGAa -3'
miRNA:   3'- -AGGUUUGGAC--GAGC-AGAUGgCCGUCU- -5'
16987 3' -54.2 NC_004323.1 + 66615 0.68 0.860925
Target:  5'- gUCCAGuaaacGCUCGUCcaugggauaaagACCGGCAGAc -3'
miRNA:   3'- -AGGUUugga-CGAGCAGa-----------UGGCCGUCU- -5'
16987 3' -54.2 NC_004323.1 + 122581 0.7 0.755696
Target:  5'- -aCGGACCgGCggCGaacUCUGCCGGUAGAc -3'
miRNA:   3'- agGUUUGGaCGa-GC---AGAUGGCCGUCU- -5'
16987 3' -54.2 NC_004323.1 + 122408 1.1 0.002984
Target:  5'- aUCCAAACCUGCUCGUCUACCGGCAGAg -3'
miRNA:   3'- -AGGUUUGGACGAGCAGAUGGCCGUCU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.