miRNA: 3'- gCAAACUGCC--CGCuGCACaUACAAca -5'
miRNA: 3'- gCAAACUGCCCGCUGCaCAuacAACa -5'
miRNA: 3'- gcaaaCuGCCCGCUGCACA---------UACAACA- -5'
miRNA: 3'- -GCAAACUGCCCGCUGCACAUACAACA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16987 | 5' | -49.5 | NC_004323.1 | + | 67160 | 0.68 | 0.986573 |
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Target: 5'- uGUUUGACGGcaGCG-CGUGcGUGUUa- -3' miRNA: 3'- gCAAACUGCC--CGCuGCACaUACAAca -5' |
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| 16987 | 5' | -49.5 | NC_004323.1 | + | 14703 | 0.69 | 0.978415 |
|
Target: 5'- gGUUUGAUGGGUGACG-GUucuUUGg -3' miRNA: 3'- gCAAACUGCCCGCUGCaCAuacAACa -5' |
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| 16987 | 5' | -49.5 | NC_004323.1 | + | 57718 | 0.74 | 0.825345 |
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Target: 5'- ----aGuCGGGCGACGUGUuugauaaaaAUGUUGUa -3' miRNA: 3'- gcaaaCuGCCCGCUGCACA---------UACAACA- -5' |
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| 16987 | 5' | -49.5 | NC_004323.1 | + | 122448 | 1.08 | 0.013278 |
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Target: 5'- cCGUUUGACGGGCGACGUGUAUGUUGUu -3' miRNA: 3'- -GCAAACUGCCCGCUGCACAUACAACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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