miRNA display CGI


Results 1 - 11 of 11 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
16991 3' -53.7 NC_004333.2 + 24991 0.66 0.77451
Target:  5'- uAUCAcgaUUCCGCAaaacGGCGcacCGACCGacgUGACg -3'
miRNA:   3'- -UAGU---AAGGCGU----CUGCa--GCUGGC---ACUG- -5'
16991 3' -53.7 NC_004333.2 + 33803 0.66 0.77451
Target:  5'- gAUCGaugCUGCGGACG-CGAuauuacCCGUGAUg -3'
miRNA:   3'- -UAGUaa-GGCGUCUGCaGCU------GGCACUG- -5'
16991 3' -53.7 NC_004333.2 + 44564 0.66 0.76415
Target:  5'- -aCGgcCCGCAGGCGccCGACUG-GGCg -3'
miRNA:   3'- uaGUaaGGCGUCUGCa-GCUGGCaCUG- -5'
16991 3' -53.7 NC_004333.2 + 43118 0.66 0.753647
Target:  5'- uUCAgcgCCGCGccGACGUCGGCCa---- -3'
miRNA:   3'- uAGUaa-GGCGU--CUGCAGCUGGcacug -5'
16991 3' -53.7 NC_004333.2 + 19219 0.67 0.68945
Target:  5'- -gCGUUCCGCAcGGCGcUCGAgcaauacgggcuggaCGUGACg -3'
miRNA:   3'- uaGUAAGGCGU-CUGC-AGCUg--------------GCACUG- -5'
16991 3' -53.7 NC_004333.2 + 37509 0.67 0.688337
Target:  5'- -gCGUgcaCCgGCcGGCG-CGACCGUGACg -3'
miRNA:   3'- uaGUAa--GG-CGuCUGCaGCUGGCACUG- -5'
16991 3' -53.7 NC_004333.2 + 8686 0.67 0.677184
Target:  5'- -gCGUUCaGCAcGGCGaacgugcCGACCGUGACg -3'
miRNA:   3'- uaGUAAGgCGU-CUGCa------GCUGGCACUG- -5'
16991 3' -53.7 NC_004333.2 + 45845 0.68 0.654754
Target:  5'- gGUCGgugCUGCucGAUGUCGAUCGUGuCg -3'
miRNA:   3'- -UAGUaa-GGCGu-CUGCAGCUGGCACuG- -5'
16991 3' -53.7 NC_004333.2 + 46133 0.72 0.37829
Target:  5'- cUCuaUCgCGCGGGCGUCGacgugaucgaugcgGCCGUGACg -3'
miRNA:   3'- uAGuaAG-GCGUCUGCAGC--------------UGGCACUG- -5'
16991 3' -53.7 NC_004333.2 + 16465 0.74 0.297519
Target:  5'- cGUCGagaUUCCGCAGaucGCGUCGAgCGUcGACg -3'
miRNA:   3'- -UAGU---AAGGCGUC---UGCAGCUgGCA-CUG- -5'
16991 3' -53.7 NC_004333.2 + 47886 0.97 0.007889
Target:  5'- cAUCAUUCCGCAGACGUCG-CCGUGACc -3'
miRNA:   3'- -UAGUAAGGCGUCUGCAGCuGGCACUG- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.