Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16994 | 3' | -51.5 | NC_004333.2 | + | 17857 | 0.66 | 0.878057 |
Target: 5'- gCCGCGcguauggCGGGUGgugucGCAGgcGCGACGg -3' miRNA: 3'- -GGCGCua-----GCCUAUa----CGUCa-CGUUGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 7483 | 0.66 | 0.875668 |
Target: 5'- gCgGCGAUCGGGUugGUGUucuuugcggucgcgAGUGCGcCGa -3' miRNA: 3'- -GgCGCUAGCCUA--UACG--------------UCACGUuGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 25154 | 0.66 | 0.861685 |
Target: 5'- aCGCuGA-CGGcaGUGCAGgcggGCGGCGUg -3' miRNA: 3'- gGCG-CUaGCCuaUACGUCa---CGUUGCA- -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 21530 | 0.66 | 0.853112 |
Target: 5'- gCGCGAUCacgcucacGAgcgGCGGUGCGACa- -3' miRNA: 3'- gGCGCUAGc-------CUauaCGUCACGUUGca -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 9291 | 0.67 | 0.835237 |
Target: 5'- gCGCGAagcggcgCGGGUGcGCGGacggGCGGCGUu -3' miRNA: 3'- gGCGCUa------GCCUAUaCGUCa---CGUUGCA- -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 27435 | 0.67 | 0.835237 |
Target: 5'- gCCGCG-UCGGcgAUGUccgGCAGCGc -3' miRNA: 3'- -GGCGCuAGCCuaUACGucaCGUUGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 19131 | 0.67 | 0.825954 |
Target: 5'- gCGCGAUCGuGGgcggcGCGGUGCucGCGc -3' miRNA: 3'- gGCGCUAGC-CUaua--CGUCACGu-UGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 14806 | 0.67 | 0.825954 |
Target: 5'- aCGCGAUCGGGca-GCAG-GCGuuccACGa -3' miRNA: 3'- gGCGCUAGCCUauaCGUCaCGU----UGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 5955 | 0.67 | 0.816454 |
Target: 5'- gCGCGA-CGGGUuuUGCAGUGgGGUGUa -3' miRNA: 3'- gGCGCUaGCCUAu-ACGUCACgUUGCA- -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 30648 | 0.68 | 0.76609 |
Target: 5'- gCGCGAUCGcuUcgGaCAGUGCGACc- -3' miRNA: 3'- gGCGCUAGCcuAuaC-GUCACGUUGca -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 4296 | 0.71 | 0.621898 |
Target: 5'- gUGCGAcaaGGAUuUGCAGUGCcGCGa -3' miRNA: 3'- gGCGCUag-CCUAuACGUCACGuUGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 40500 | 0.71 | 0.610553 |
Target: 5'- gCCGUGGUCGGGUucgccgGCAGUcgaaGCGGuCGUg -3' miRNA: 3'- -GGCGCUAGCCUAua----CGUCA----CGUU-GCA- -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 11979 | 0.71 | 0.599228 |
Target: 5'- gUCGaCGAUCGcGGccgUAUcGCGGUGCAACGg -3' miRNA: 3'- -GGC-GCUAGC-CU---AUA-CGUCACGUUGCa -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 23485 | 0.75 | 0.37101 |
Target: 5'- gCGCGcgCGGAUcggcAUGCAGUugaucggcGCAGCGUa -3' miRNA: 3'- gGCGCuaGCCUA----UACGUCA--------CGUUGCA- -5' |
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16994 | 3' | -51.5 | NC_004333.2 | + | 47024 | 1.09 | 0.002153 |
Target: 5'- gCCGCGAUCGGAUAUGCAGUGCAACGUc -3' miRNA: 3'- -GGCGCUAGCCUAUACGUCACGUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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