Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16995 | 3' | -54 | NC_004333.2 | + | 35165 | 0.66 | 0.782521 |
Target: 5'- cGCA--GGCGUUGAugccCGGCGCGaacgucgauUUGUCGa -3' miRNA: 3'- -CGUacCCGUAGCU----GCCGUGC---------AACAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 4946 | 0.66 | 0.762141 |
Target: 5'- gGCGcGGGCAUgCGAacgcaCGGCACGagGUUc -3' miRNA: 3'- -CGUaCCCGUA-GCU-----GCCGUGCaaCAGc -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 30707 | 0.66 | 0.751742 |
Target: 5'- aGCGUGaGCG-CGGCGGCcuucGCGUUG-CGc -3' miRNA: 3'- -CGUACcCGUaGCUGCCG----UGCAACaGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 32279 | 0.67 | 0.73058 |
Target: 5'- cGgAUGGcGCggGUCGGCGGCGa--UGUCGa -3' miRNA: 3'- -CgUACC-CG--UAGCUGCCGUgcaACAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 4257 | 0.67 | 0.73058 |
Target: 5'- aCAUGGGUAUCGGUGcGCuACGUggcguuugGUCGc -3' miRNA: 3'- cGUACCCGUAGCUGC-CG-UGCAa-------CAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 39747 | 0.67 | 0.719842 |
Target: 5'- ---cGGGCAgaucaacgUCGGCGGCaccguaucggcgACGUUGcUCGa -3' miRNA: 3'- cguaCCCGU--------AGCUGCCG------------UGCAAC-AGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 11899 | 0.67 | 0.709015 |
Target: 5'- cGCAUGGcGCAg-GACGuCGCGU-GUCGc -3' miRNA: 3'- -CGUACC-CGUagCUGCcGUGCAaCAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 21885 | 0.67 | 0.687141 |
Target: 5'- gGCuuucGCAUCGACGGCGCGgUG-CGc -3' miRNA: 3'- -CGuaccCGUAGCUGCCGUGCaACaGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 34586 | 0.67 | 0.676118 |
Target: 5'- cGCAcuugGGGCGaagcgaUCGGCGGCACGa----- -3' miRNA: 3'- -CGUa---CCCGU------AGCUGCCGUGCaacagc -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 29302 | 0.68 | 0.653955 |
Target: 5'- uGCGuucUGGGCGcccCGGCGGCaauGCGUUG-CGc -3' miRNA: 3'- -CGU---ACCCGUa--GCUGCCG---UGCAACaGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 43325 | 0.69 | 0.57627 |
Target: 5'- gGCAUcGGCA-CGGCGcGCGCGUacgGUCa -3' miRNA: 3'- -CGUAcCCGUaGCUGC-CGUGCAa--CAGc -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 28775 | 0.69 | 0.57627 |
Target: 5'- cGCGcGGGUcgCGGCGGCGCGa----- -3' miRNA: 3'- -CGUaCCCGuaGCUGCCGUGCaacagc -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 7117 | 0.7 | 0.554361 |
Target: 5'- aCGUGcGG-AUUGGCGGCGCGaaacgUGUCGa -3' miRNA: 3'- cGUAC-CCgUAGCUGCCGUGCa----ACAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 14588 | 0.7 | 0.543497 |
Target: 5'- aCAUGGGaCAgcggcuUCGGCuGCugGUUGUUGa -3' miRNA: 3'- cGUACCC-GU------AGCUGcCGugCAACAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 45241 | 0.7 | 0.500818 |
Target: 5'- uGCA-GGGCuacaGCGGCACGUUGaCGg -3' miRNA: 3'- -CGUaCCCGuagcUGCCGUGCAACaGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 46139 | 0.71 | 0.459681 |
Target: 5'- cGCGcGGGCGUCGacgugaucgauGCGGC-CGUgacGUCGg -3' miRNA: 3'- -CGUaCCCGUAGC-----------UGCCGuGCAa--CAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 7184 | 0.75 | 0.278285 |
Target: 5'- -gGUGGGCuucGUCGACGaGCACGaaGUCGa -3' miRNA: 3'- cgUACCCG---UAGCUGC-CGUGCaaCAGC- -5' |
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16995 | 3' | -54 | NC_004333.2 | + | 46601 | 1.12 | 0.000768 |
Target: 5'- aGCAUGGGCAUCGACGGCACGUUGUCGg -3' miRNA: 3'- -CGUACCCGUAGCUGCCGUGCAACAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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