Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16995 | 5' | -54.2 | NC_004333.2 | + | 33862 | 0.66 | 0.72493 |
Target: 5'- -gCGGCGAacGGUGCCGCgc-UGAUCGa -3' miRNA: 3'- aaGUUGCU--CCACGGCGacuGCUAGUg -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 29801 | 0.66 | 0.713931 |
Target: 5'- -gCGACGAcGGUcaccggGCCGUUGAugcUGAUCGCc -3' miRNA: 3'- aaGUUGCU-CCA------CGGCGACU---GCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 258 | 0.66 | 0.713931 |
Target: 5'- gUUCGAUGuGGUcgucacGCCGCcGAgcaCGGUCACg -3' miRNA: 3'- -AAGUUGCuCCA------CGGCGaCU---GCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 16001 | 0.66 | 0.713931 |
Target: 5'- gUCAACGAGGU-CUGC-GACGA--GCg -3' miRNA: 3'- aAGUUGCUCCAcGGCGaCUGCUagUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 17411 | 0.66 | 0.713931 |
Target: 5'- -gCAGCGcGGUcacGaCgCGCUGACGAUCAa -3' miRNA: 3'- aaGUUGCuCCA---C-G-GCGACUGCUAGUg -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 36055 | 0.66 | 0.68047 |
Target: 5'- -aCGGCGAGcucGUGCCGCUcgguaaguucGACGGguucuuUCACg -3' miRNA: 3'- aaGUUGCUC---CACGGCGA----------CUGCU------AGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 18270 | 0.66 | 0.68047 |
Target: 5'- -gCGGcCGAGGaUGCCGCgcgcGGCccGAUCGCg -3' miRNA: 3'- aaGUU-GCUCC-ACGGCGa---CUG--CUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 10799 | 0.66 | 0.669202 |
Target: 5'- -aCGAUGAGGacgGCCGCgcGACGcggugcacGUCGCg -3' miRNA: 3'- aaGUUGCUCCa--CGGCGa-CUGC--------UAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 1729 | 0.67 | 0.646567 |
Target: 5'- -cCGACGAGcGUGuCCGCUGGuacCGGaCACu -3' miRNA: 3'- aaGUUGCUC-CAC-GGCGACU---GCUaGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 35482 | 0.67 | 0.623876 |
Target: 5'- gUCAACGGGccggugagcGUGCCGCcuGCGAgcggCACg -3' miRNA: 3'- aAGUUGCUC---------CACGGCGacUGCUa---GUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 19274 | 0.68 | 0.556306 |
Target: 5'- -cCGGCGAccuGGUGCUGCUcGCGAgcaagCACa -3' miRNA: 3'- aaGUUGCU---CCACGGCGAcUGCUa----GUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 24737 | 0.68 | 0.556306 |
Target: 5'- aUCGGuCGAGaaGCCGCUcacgaacgcggcGACGAUCGCu -3' miRNA: 3'- aAGUU-GCUCcaCGGCGA------------CUGCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 41730 | 0.69 | 0.545218 |
Target: 5'- aUCAACGAGGagaucGgCGCgGGCGAcUCGCg -3' miRNA: 3'- aAGUUGCUCCa----CgGCGaCUGCU-AGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 3547 | 0.69 | 0.491006 |
Target: 5'- aUCAACGcGGUGCCGCgcaGCGcGUCGg -3' miRNA: 3'- aAGUUGCuCCACGGCGac-UGC-UAGUg -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 12703 | 0.7 | 0.459699 |
Target: 5'- cUCAcCGGcccguugacGGUGCCGacguUGACGGUCACg -3' miRNA: 3'- aAGUuGCU---------CCACGGCg---ACUGCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 33293 | 0.7 | 0.4495 |
Target: 5'- -aCGACucGGUGCUGCUGACGcgCcuGCa -3' miRNA: 3'- aaGUUGcuCCACGGCGACUGCuaG--UG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 7449 | 0.7 | 0.4495 |
Target: 5'- gUUCAggaGCGAcuUGCCGCcgccggUGACGAUCGCg -3' miRNA: 3'- -AAGU---UGCUccACGGCG------ACUGCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 37061 | 0.72 | 0.354989 |
Target: 5'- -cCGGCGAGGcgauuucgUGCCGCUGuucgucgcgcACGAUCAUu -3' miRNA: 3'- aaGUUGCUCC--------ACGGCGAC----------UGCUAGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 15620 | 0.74 | 0.28265 |
Target: 5'- uUUCGGCGAacgucGUGCCGUUGGCGAccucgaUCACg -3' miRNA: 3'- -AAGUUGCUc----CACGGCGACUGCU------AGUG- -5' |
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16995 | 5' | -54.2 | NC_004333.2 | + | 46639 | 1.08 | 0.00107 |
Target: 5'- gUUCAACGAGGUGCCGCUGACGAUCACg -3' miRNA: 3'- -AAGUUGCUCCACGGCGACUGCUAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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