Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16996 | 3' | -53.2 | NC_004333.2 | + | 27928 | 0.66 | 0.803032 |
Target: 5'- uCGAUGUGCgGCGCGAUGuAGGC-Ca- -3' miRNA: 3'- -GCUACAUGaCGUGCUGCcUUCGcGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 17874 | 0.66 | 0.793133 |
Target: 5'- uGGUGUcGCagGCGCGACGGcGGCcaGCUc -3' miRNA: 3'- gCUACA-UGa-CGUGCUGCCuUCG--CGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 29888 | 0.66 | 0.793133 |
Target: 5'- gCGAaGcGCUGCGCGAuCGGGccGCGCg- -3' miRNA: 3'- -GCUaCaUGACGUGCU-GCCUu-CGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 47809 | 0.66 | 0.793133 |
Target: 5'- uGAUcGUGCaGCGCGccgccuGCGGAcGGCGCUu -3' miRNA: 3'- gCUA-CAUGaCGUGC------UGCCU-UCGCGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 3304 | 0.66 | 0.772817 |
Target: 5'- --uUGUGC-GCGCGGCGGc-GCGCg- -3' miRNA: 3'- gcuACAUGaCGUGCUGCCuuCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 41770 | 0.66 | 0.772817 |
Target: 5'- -uAUGgguCUGCACGAgcgaGGAAGUGCg- -3' miRNA: 3'- gcUACau-GACGUGCUg---CCUUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 46471 | 0.66 | 0.762425 |
Target: 5'- gGAUacGUACUGCGCGACuauuacaaaGGcugcAAGCGCg- -3' miRNA: 3'- gCUA--CAUGACGUGCUG---------CC----UUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 34542 | 0.67 | 0.751893 |
Target: 5'- cCGA-GUACcGCGCGAUGcacGAAGUGCUc -3' miRNA: 3'- -GCUaCAUGaCGUGCUGC---CUUCGCGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 13356 | 0.67 | 0.751893 |
Target: 5'- aCGGUGUACggGCGCGGCacGAgcGGCGUg- -3' miRNA: 3'- -GCUACAUGa-CGUGCUGc-CU--UCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 48067 | 0.67 | 0.751893 |
Target: 5'- gCGAUGUcguGCUGCucggugcCGACG-AGGCGCa- -3' miRNA: 3'- -GCUACA---UGACGu------GCUGCcUUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 27380 | 0.67 | 0.741232 |
Target: 5'- gGAaGUGCccgGCGCGACGGccGUGCc- -3' miRNA: 3'- gCUaCAUGa--CGUGCUGCCuuCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 36338 | 0.67 | 0.719577 |
Target: 5'- gCGAUGUACcgGCGCaaGCuGGAGCGCg- -3' miRNA: 3'- -GCUACAUGa-CGUGc-UGcCUUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 16673 | 0.67 | 0.719577 |
Target: 5'- cCGGUGcUGCaccGCGCGguGCGGAAGCaccGCUGc -3' miRNA: 3'- -GCUAC-AUGa--CGUGC--UGCCUUCG---CGAC- -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 44379 | 0.68 | 0.686443 |
Target: 5'- aGGUGcGCUGCuCGGCGGcgcguGCGCg- -3' miRNA: 3'- gCUACaUGACGuGCUGCCuu---CGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 8941 | 0.68 | 0.66406 |
Target: 5'- uCGggGUGCggucgagcauUGCAcgcagcucggcCGACGGGAGCGCg- -3' miRNA: 3'- -GCuaCAUG----------ACGU-----------GCUGCCUUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 42955 | 0.68 | 0.649438 |
Target: 5'- gGAUGcGCUGCAacccgcacccucgcCGGCGGAcgagcgggcGGCGCUc -3' miRNA: 3'- gCUACaUGACGU--------------GCUGCCU---------UCGCGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 770 | 0.69 | 0.630279 |
Target: 5'- gCGAUGUAgcugcgauugcUUGCGCGuCGGucGGCGCUu -3' miRNA: 3'- -GCUACAU-----------GACGUGCuGCCu-UCGCGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 37797 | 0.7 | 0.563027 |
Target: 5'- gGAUGcgaaGCgGCGCGAUGcGAAGCGCg- -3' miRNA: 3'- gCUACa---UGaCGUGCUGC-CUUCGCGac -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 1658 | 0.71 | 0.519233 |
Target: 5'- -aAUGcGCUGCGCGGuCGGAaacAGCGCUu -3' miRNA: 3'- gcUACaUGACGUGCU-GCCU---UCGCGAc -5' |
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16996 | 3' | -53.2 | NC_004333.2 | + | 5517 | 0.73 | 0.38842 |
Target: 5'- gCGcUGUugcggGCUGCuCGACGGGAGaCGCUGc -3' miRNA: 3'- -GCuACA-----UGACGuGCUGCCUUC-GCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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