Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16998 | 5' | -54.4 | NC_004333.2 | + | 21549 | 0.65 | 0.776529 |
Target: 5'- cGGCGGUgcgacaacgaucaACGCCGccaGCUUUGUCGUGa -3' miRNA: 3'- cCUGCCAa------------UGUGGCc--CGAGAUAGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 2948 | 0.66 | 0.774515 |
Target: 5'- cGGACaGGUcgGCaccgacaagcacgugGCCGGGCUUcguggCGCGg -3' miRNA: 3'- -CCUG-CCAa-UG---------------UGGCCCGAGaua--GCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 35781 | 0.66 | 0.770471 |
Target: 5'- aGGCGGcgacacuggcGCGCCGGGCgggg-CGCGg -3' miRNA: 3'- cCUGCCaa--------UGUGGCCCGagauaGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 41749 | 0.66 | 0.749919 |
Target: 5'- cGGGCGacucgcGCGCCGGGCUauggGUCuGCa -3' miRNA: 3'- -CCUGCcaa---UGUGGCCCGAga--UAG-CGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 44967 | 0.67 | 0.718216 |
Target: 5'- gGGGCGGgcggcGCGCCGGGCcggCUcaccggccUCGUGc -3' miRNA: 3'- -CCUGCCaa---UGUGGCCCGa--GAu-------AGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 8304 | 0.68 | 0.663814 |
Target: 5'- aGGCGGc-GCGCgGGGCUCg--CGCc -3' miRNA: 3'- cCUGCCaaUGUGgCCCGAGauaGCGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 39539 | 0.68 | 0.630725 |
Target: 5'- cGGGCGcc-GCGCCGaGCUCgacGUCGCGc -3' miRNA: 3'- -CCUGCcaaUGUGGCcCGAGa--UAGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 31631 | 0.68 | 0.619685 |
Target: 5'- uGACGG--ACGCCGGGCa--GUUGCGc -3' miRNA: 3'- cCUGCCaaUGUGGCCCGagaUAGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 29805 | 0.68 | 0.619685 |
Target: 5'- cGACGGU--CACCGGGCcguugaugCUgAUCGCc -3' miRNA: 3'- cCUGCCAauGUGGCCCGa-------GA-UAGCGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 32298 | 0.69 | 0.586667 |
Target: 5'- uGGCGGU--CACgGcGGCUCgcUCGCGg -3' miRNA: 3'- cCUGCCAauGUGgC-CCGAGauAGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 2796 | 0.69 | 0.575725 |
Target: 5'- -aGCGGUUGCGCaGGGCaUCguaCGCGa -3' miRNA: 3'- ccUGCCAAUGUGgCCCG-AGauaGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 15708 | 0.69 | 0.553991 |
Target: 5'- --cCGGUUACauGCUGcGGCUC-GUCGCGg -3' miRNA: 3'- ccuGCCAAUG--UGGC-CCGAGaUAGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 41585 | 0.7 | 0.520817 |
Target: 5'- uGGGCGGc-GCGCCguggcggcgaugcGGGCUUUcgCGCGu -3' miRNA: 3'- -CCUGCCaaUGUGG-------------CCCGAGAuaGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 38431 | 0.7 | 0.490507 |
Target: 5'- cGACGGgcgcGCAuCCGGcGCUCaUGUCGCc -3' miRNA: 3'- cCUGCCaa--UGU-GGCC-CGAG-AUAGCGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 27290 | 0.72 | 0.421052 |
Target: 5'- -cACGcGcUGCGCCGGGCUC-GUCGCc -3' miRNA: 3'- ccUGC-CaAUGUGGCCCGAGaUAGCGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 31513 | 0.75 | 0.294183 |
Target: 5'- -cGCGGUgcaGCACCGGGCaggucgaagugCUGUCGCa -3' miRNA: 3'- ccUGCCAa--UGUGGCCCGa----------GAUAGCGc -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 37421 | 0.76 | 0.246007 |
Target: 5'- --uCGGUUGCGCCGGGC-Cg--CGCGg -3' miRNA: 3'- ccuGCCAAUGUGGCCCGaGauaGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 17036 | 0.79 | 0.160273 |
Target: 5'- cGGGCGGcgaugcauuCGCCGGGCUCgcgCGCGa -3' miRNA: 3'- -CCUGCCaau------GUGGCCCGAGauaGCGC- -5' |
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16998 | 5' | -54.4 | NC_004333.2 | + | 46116 | 1.11 | 0.00097 |
Target: 5'- aGGACGGUUACACCGGGCUCUAUCGCGc -3' miRNA: 3'- -CCUGCCAAUGUGGCCCGAGAUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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