Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
170 | 3' | -60 | AC_000007.1 | + | 19000 | 0.66 | 0.380404 |
Target: 5'- cCCAGCGUUugACGCUGcGGUuc-AUCCCu -3' miRNA: 3'- aGGUCGCGG--UGCGAC-CCGcaaUGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 24002 | 0.66 | 0.354446 |
Target: 5'- aCC-GCGuCCGCGCUcGGGgGUgguuucgcgcUGCUCCu -3' miRNA: 3'- aGGuCGC-GGUGCGA-CCCgCA----------AUGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 7293 | 0.66 | 0.354446 |
Target: 5'- cUCCGGaagGCCGCGCaGGcauacGCGcUACCCg -3' miRNA: 3'- -AGGUCg--CGGUGCGaCC-----CGCaAUGGGg -5' |
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170 | 3' | -60 | AC_000007.1 | + | 13312 | 0.66 | 0.337848 |
Target: 5'- cCCAGCGUgGCGCUGGaCaugACCgCg -3' miRNA: 3'- aGGUCGCGgUGCGACCcGcaaUGGgG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 18887 | 0.67 | 0.329763 |
Target: 5'- aCCugAGCcCCGgGCUGGuGCaGUUugCCCg -3' miRNA: 3'- aGG--UCGcGGUgCGACC-CG-CAAugGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 8698 | 0.67 | 0.306371 |
Target: 5'- cUCCuGC-CCGCGC-GcGGCGccgacgUGCCCCu -3' miRNA: 3'- -AGGuCGcGGUGCGaC-CCGCa-----AUGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 16006 | 0.67 | 0.304103 |
Target: 5'- gCCcGCGCaCGCGCacUGGGUGUuggucgagcgcuucUuuGCCCCg -3' miRNA: 3'- aGGuCGCG-GUGCG--ACCCGCA--------------A--UGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 8640 | 0.68 | 0.27719 |
Target: 5'- gUCGGCGCCGCGCgcGGGCaGgagcuggUGCUgCg -3' miRNA: 3'- aGGUCGCGGUGCGa-CCCG-Ca------AUGGgG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 11229 | 0.68 | 0.263451 |
Target: 5'- cCCGGCGCCGCGg-GGGUucGUaaucaccaucUGCCgCCg -3' miRNA: 3'- aGGUCGCGGUGCgaCCCG--CA----------AUGG-GG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 12244 | 0.68 | 0.25027 |
Target: 5'- gCCAGCG-CGCGC-GGGUGccACCgCCa -3' miRNA: 3'- aGGUCGCgGUGCGaCCCGCaaUGG-GG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 13736 | 0.7 | 0.197582 |
Target: 5'- gCCcGCGCC-UGCUGGGCGaggaggagUACCUa -3' miRNA: 3'- aGGuCGCGGuGCGACCCGCa-------AUGGGg -5' |
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170 | 3' | -60 | AC_000007.1 | + | 14197 | 0.7 | 0.197582 |
Target: 5'- -gCGGCGCCaguggcggcgGCGCUGGGUu---CCCCc -3' miRNA: 3'- agGUCGCGG----------UGCGACCCGcaauGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 16259 | 0.71 | 0.172692 |
Target: 5'- gCCGcCGCCGCGCguUGGGCGgcagUGCCg- -3' miRNA: 3'- aGGUcGCGGUGCG--ACCCGCa---AUGGgg -5' |
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170 | 3' | -60 | AC_000007.1 | + | 22723 | 0.72 | 0.150639 |
Target: 5'- aCUcGCG-CGCGCaGGGCGgagGCCCCa -3' miRNA: 3'- aGGuCGCgGUGCGaCCCGCaa-UGGGG- -5' |
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170 | 3' | -60 | AC_000007.1 | + | 4264 | 0.76 | 0.074441 |
Target: 5'- aUCCAGuCGUagcaggaGCGCUGGGCGUggUGCCUa -3' miRNA: 3'- -AGGUC-GCGg------UGCGACCCGCA--AUGGGg -5' |
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170 | 3' | -60 | AC_000007.1 | + | 13364 | 1.12 | 0.000115 |
Target: 5'- gUCCAGCGCCACGCUGGGCGUUACCCCg -3' miRNA: 3'- -AGGUCGCGGUGCGACCCGCAAUGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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