Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17002 | 5' | -55 | NC_004333.2 | + | 39480 | 0.66 | 0.701809 |
Target: 5'- cGGcGUCAcGGUCGGCacguucgccgUGCUgaacgccgACGGCaCCg -3' miRNA: 3'- aCCaUAGU-CCAGCUG----------ACGA--------UGCCG-GG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 23368 | 0.66 | 0.668848 |
Target: 5'- -cGUG-CAGGUCGcgcaGCUGCcgaACGuGCCCg -3' miRNA: 3'- acCAUaGUCCAGC----UGACGa--UGC-CGGG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 17873 | 0.67 | 0.64668 |
Target: 5'- gUGGUGUCgcAGGcgCGACg---GCGGCCa -3' miRNA: 3'- -ACCAUAG--UCCa-GCUGacgaUGCCGGg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 13348 | 0.67 | 0.624457 |
Target: 5'- ---cGUCAGGgauUCGACUGCUgGCgGGCaCCu -3' miRNA: 3'- accaUAGUCC---AGCUGACGA-UG-CCG-GG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 41637 | 0.67 | 0.624457 |
Target: 5'- cGGcagauUGUCGGG-CG-CggGCUGCGGCCg -3' miRNA: 3'- aCC-----AUAGUCCaGCuGa-CGAUGCCGGg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 36753 | 0.68 | 0.602262 |
Target: 5'- cGGUcgaAGG-CGACgUGCUGCGGCa- -3' miRNA: 3'- aCCAuagUCCaGCUG-ACGAUGCCGgg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 33156 | 0.68 | 0.569186 |
Target: 5'- aGGUGgcgaguuucgCGGcGUCGGCgucgGCGGCCCg -3' miRNA: 3'- aCCAUa---------GUC-CAGCUGacgaUGCCGGG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 32244 | 0.68 | 0.558254 |
Target: 5'- cGGaUAUC-GGUUGGCaGCaGCGGCCg -3' miRNA: 3'- aCC-AUAGuCCAGCUGaCGaUGCCGGg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 22498 | 0.69 | 0.536578 |
Target: 5'- aGGcugAUCAGGUCGccGCguucGCgACGGCCg -3' miRNA: 3'- aCCa--UAGUCCAGC--UGa---CGaUGCCGGg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 15698 | 0.69 | 0.536578 |
Target: 5'- cUGGUGUUguccGGUUacaUGCUGCGGCUCg -3' miRNA: 3'- -ACCAUAGu---CCAGcugACGAUGCCGGG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 40644 | 0.69 | 0.504644 |
Target: 5'- cUGGUAUCAGGcCGAa----GCGGCCg -3' miRNA: 3'- -ACCAUAGUCCaGCUgacgaUGCCGGg -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 13720 | 0.72 | 0.351585 |
Target: 5'- aUGGUAuUCAGGUCuaugaUGCggGCGGCCUc -3' miRNA: 3'- -ACCAU-AGUCCAGcug--ACGa-UGCCGGG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 22189 | 0.72 | 0.351585 |
Target: 5'- cGGcggCGGuUCGGCgGCUGCGGCCUg -3' miRNA: 3'- aCCauaGUCcAGCUGaCGAUGCCGGG- -5' |
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17002 | 5' | -55 | NC_004333.2 | + | 44544 | 1.13 | 0.000514 |
Target: 5'- cUGGUAUCAGGUCGACUGCUACGGCCCg -3' miRNA: 3'- -ACCAUAGUCCAGCUGACGAUGCCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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