Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17004 | 5' | -52.6 | NC_004333.2 | + | 46344 | 0.74 | 0.345199 |
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Target: 5'- gCGGGCGcCGuuAUGCGCcGCUGCGGAUUu -3' miRNA: 3'- -GCUCGCaGU--UGCGCGuCGACGUUUAG- -5' |
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| 17004 | 5' | -52.6 | NC_004333.2 | + | 2268 | 0.76 | 0.273897 |
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Target: 5'- uCGAGCG-CAGCGCGUuuGCcgucgGCAAGUCg -3' miRNA: 3'- -GCUCGCaGUUGCGCGu-CGa----CGUUUAG- -5' |
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| 17004 | 5' | -52.6 | NC_004333.2 | + | 4766 | 0.79 | 0.162452 |
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Target: 5'- cCGAGCGUCugccuGCGCggGCGGCUGCGGGa- -3' miRNA: 3'- -GCUCGCAGu----UGCG--CGUCGACGUUUag -5' |
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| 17004 | 5' | -52.6 | NC_004333.2 | + | 3688 | 0.8 | 0.149122 |
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Target: 5'- uGAGcCGUC-GCGuCGUAGCUGCGGAUCg -3' miRNA: 3'- gCUC-GCAGuUGC-GCGUCGACGUUUAG- -5' |
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| 17004 | 5' | -52.6 | NC_004333.2 | + | 45219 | 0.9 | 0.032527 |
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Target: 5'- aCGGGCGUCAcgcuCGCGCAGCUGCAGGg- -3' miRNA: 3'- -GCUCGCAGUu---GCGCGUCGACGUUUag -5' |
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| 17004 | 5' | -52.6 | NC_004333.2 | + | 43961 | 1.09 | 0.001386 |
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Target: 5'- uCGAGCGUCAACGCGCAGCUGCAAAUCc -3' miRNA: 3'- -GCUCGCAGUUGCGCGUCGACGUUUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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