Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17005 | 3' | -52.4 | NC_004333.2 | + | 40315 | 0.66 | 0.820606 |
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Target: 5'- uGggGCGAgcgcgagcuGGaaaUGGCcgGCGCAggccgcgucgaucugGCGAGCg -3' miRNA: 3'- gCuuCGCU---------UC---ACCGa-CGCGU---------------UGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 41297 | 0.66 | 0.816767 |
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Target: 5'- uCGccGCGAuccgcucgaUGGCUGCGCAAUGcaguGCg -3' miRNA: 3'- -GCuuCGCUuc-------ACCGACGCGUUGCu---UG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 41305 | 0.69 | 0.655573 |
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Target: 5'- aCGAGGCcGAGcucgcccGGC-GCGCGAUGGACg -3' miRNA: 3'- -GCUUCGcUUCa------CCGaCGCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 41588 | 0.67 | 0.755596 |
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Target: 5'- gCGgcGCGccGUGGCgGCGauGCGGGCu -3' miRNA: 3'- -GCuuCGCuuCACCGaCGCguUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 42081 | 0.74 | 0.378742 |
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Target: 5'- aCGAggauAGCGucGU-GCUGCGCGACGAGg -3' miRNA: 3'- -GCU----UCGCuuCAcCGACGCGUUGCUUg -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 42712 | 1.11 | 0.001092 |
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Target: 5'- cCGAAGCGAAGUGGCUGCGCAACGAACa -3' miRNA: 3'- -GCUUCGCUUCACCGACGCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 44479 | 0.72 | 0.466991 |
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Target: 5'- gGgcGCGAAGUGGaUGaaggaaauCGCAGCGAACc -3' miRNA: 3'- gCuuCGCUUCACCgAC--------GCGUUGCUUG- -5' |
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| 17005 | 3' | -52.4 | NC_004333.2 | + | 45101 | 0.67 | 0.764093 |
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Target: 5'- gCGAGGCGAuacucgacgccUGuCUGCGCGGCGAucGCg -3' miRNA: 3'- -GCUUCGCUuc---------ACcGACGCGUUGCU--UG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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