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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17007 | 3' | -52.5 | NC_004333.2 | + | 12522 | 0.67 | 0.726646 |
Target: 5'- cGGCUcgGUGUUcaGGCGUgccgcuaccugaUCGGGGCGg- -3' miRNA: 3'- uCUGAa-CACGA--UUGCA------------AGCCCCGCau -5' |
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17007 | 3' | -52.5 | NC_004333.2 | + | 5702 | 0.71 | 0.501614 |
Target: 5'- uGACUUGUGaaaUGAa-UUCGGGGCGa- -3' miRNA: 3'- uCUGAACACg--AUUgcAAGCCCCGCau -5' |
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17007 | 3' | -52.5 | NC_004333.2 | + | 42436 | 1.07 | 0.002141 |
Target: 5'- uAGACUUGUGCUAACGUUCGGGGCGUAc -3' miRNA: 3'- -UCUGAACACGAUUGCAAGCCCCGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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