Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17007 | 5' | -46.1 | NC_004333.2 | + | 45524 | 0.66 | 0.995192 |
Target: 5'- cGGggCGCCCCGcAUUCGag-CGguGGu -3' miRNA: 3'- -CCaaGCGGGGCuUAAGUaaaGUguUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 2891 | 0.66 | 0.993303 |
Target: 5'- ---aCGCCUCGAGcUUCGggacgucgUUCGCGAGc -3' miRNA: 3'- ccaaGCGGGGCUU-AAGUa-------AAGUGUUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 3687 | 0.66 | 0.992157 |
Target: 5'- gGGUUCGCUgCGAuuuccUUCAUccacUUCGCGc- -3' miRNA: 3'- -CCAAGCGGgGCUu----AAGUA----AAGUGUuc -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 17190 | 0.67 | 0.987767 |
Target: 5'- ----aGCCCCGAucuGUUUAUcgUCGCGGGc -3' miRNA: 3'- ccaagCGGGGCU---UAAGUAa-AGUGUUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 27925 | 0.68 | 0.973255 |
Target: 5'- cGUUCGCCUCGA-------UCACAAGc -3' miRNA: 3'- cCAAGCGGGGCUuaaguaaAGUGUUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 9850 | 0.69 | 0.959066 |
Target: 5'- cGGcaCGCCCuCGAGgcgCAUUUCGCcGGu -3' miRNA: 3'- -CCaaGCGGG-GCUUaa-GUAAAGUGuUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 5736 | 0.76 | 0.693635 |
Target: 5'- gGGUaCGCCCCGAAcgUUAg--CACAAGu -3' miRNA: 3'- -CCAaGCGGGGCUUa-AGUaaaGUGUUC- -5' |
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17007 | 5' | -46.1 | NC_004333.2 | + | 42470 | 1.13 | 0.00507 |
Target: 5'- aGGUUCGCCCCGAAUUCAUUUCACAAGu -3' miRNA: 3'- -CCAAGCGGGGCUUAAGUAAAGUGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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