Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17009 | 3' | -50.2 | NC_004333.2 | + | 13004 | 0.66 | 0.928594 |
Target: 5'- aGGU---UAUGCAACUGCcgGCGAuGGCGg -3' miRNA: 3'- gCUAuacAUGCGUUGACG--CGCU-CUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 31108 | 0.66 | 0.92258 |
Target: 5'- cCGAUGUuccgGCGCGGCUGgaacacCGCGAGcAUGa -3' miRNA: 3'- -GCUAUAca--UGCGUUGAC------GCGCUC-UGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 8908 | 0.66 | 0.916267 |
Target: 5'- gCGAg--GUugGCGucgaUGCGCGAcGCGg -3' miRNA: 3'- -GCUauaCAugCGUug--ACGCGCUcUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 36113 | 0.66 | 0.915619 |
Target: 5'- -----cGUACGCAAgguuggcgacggcCUGCGCGAccGGCGc -3' miRNA: 3'- gcuauaCAUGCGUU-------------GACGCGCU--CUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 22250 | 0.66 | 0.909656 |
Target: 5'- aGAUAgcugaucGUGCGCu-CUGCGCcaagccagcccGAGACGc -3' miRNA: 3'- gCUAUa------CAUGCGuuGACGCG-----------CUCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 37724 | 0.67 | 0.895552 |
Target: 5'- uCGAUGUcGgcaagcugcCGCGcCUGCGCGAGAuCGa -3' miRNA: 3'- -GCUAUA-Cau-------GCGUuGACGCGCUCU-GC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 443 | 0.67 | 0.888067 |
Target: 5'- cCGggGUGUACGaGGCcacgGCGCGcGACGu -3' miRNA: 3'- -GCuaUACAUGCgUUGa---CGCGCuCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 46889 | 0.67 | 0.888067 |
Target: 5'- cCGAUcgcGUAaCGCAACcGCGCcAGGCGg -3' miRNA: 3'- -GCUAua-CAU-GCGUUGaCGCGcUCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 27673 | 0.67 | 0.880299 |
Target: 5'- gCGAguc--GCGCAccugaacgcGCUGCaGCGAGACGu -3' miRNA: 3'- -GCUauacaUGCGU---------UGACG-CGCUCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 4212 | 0.67 | 0.880299 |
Target: 5'- gGAUu----UGCAGCUGCGCGuuGACGc -3' miRNA: 3'- gCUAuacauGCGUUGACGCGCu-CUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 9293 | 0.67 | 0.872255 |
Target: 5'- gCGAagcg-GCGCGGgUGCGCG-GACGg -3' miRNA: 3'- -GCUauacaUGCGUUgACGCGCuCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 24456 | 0.68 | 0.855368 |
Target: 5'- aCGAcGUccgACGCAACUcGCGCGAGcCa -3' miRNA: 3'- -GCUaUAca-UGCGUUGA-CGCGCUCuGc -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 15753 | 0.69 | 0.778526 |
Target: 5'- aCGAgacacucGUcaGCG-AGCUGCGCGAGGCGc -3' miRNA: 3'- -GCUaua----CA--UGCgUUGACGCGCUCUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 13649 | 0.7 | 0.746677 |
Target: 5'- gCGGUAcucGgGCAGCUGCGCGAGcagcACGa -3' miRNA: 3'- -GCUAUacaUgCGUUGACGCGCUC----UGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 27561 | 0.7 | 0.746677 |
Target: 5'- uCGAauUGgcC-CGACUGCGCGAGGCc -3' miRNA: 3'- -GCUauACauGcGUUGACGCGCUCUGc -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 2948 | 0.7 | 0.724796 |
Target: 5'- ----cUGUAgcccUGCAGCUGCGCGAG-CGu -3' miRNA: 3'- gcuauACAU----GCGUUGACGCGCUCuGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 28927 | 0.71 | 0.691256 |
Target: 5'- -cGUcgGUGCGUAGCggGCGCcGAGGCa -3' miRNA: 3'- gcUAuaCAUGCGUUGa-CGCG-CUCUGc -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 24172 | 0.71 | 0.679937 |
Target: 5'- cCGGUA-GUACGCAuccuGCaGCGCGAaugcGACGg -3' miRNA: 3'- -GCUAUaCAUGCGU----UGaCGCGCU----CUGC- -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 8294 | 0.72 | 0.634286 |
Target: 5'- uGAUGUagGUAgGCGGC-GCGCGGGGCu -3' miRNA: 3'- gCUAUA--CAUgCGUUGaCGCGCUCUGc -5' |
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17009 | 3' | -50.2 | NC_004333.2 | + | 28740 | 0.73 | 0.599983 |
Target: 5'- aGGUcgGccaGCAACUGCGCGAG-CGc -3' miRNA: 3'- gCUAuaCaugCGUUGACGCGCUCuGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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