Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17017 | 3' | -53.7 | NC_004333.2 | + | 25939 | 0.66 | 0.805396 |
Target: 5'- gCGcgCGGCGCGAUGccgGCGGccAGUaCGc -3' miRNA: 3'- -GCaaGCCGCGCUACcuaCGCU--UCA-GC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 33650 | 0.66 | 0.795683 |
Target: 5'- aGUUCGuGCGCGugaucaugucGUGcGAUGCGAcGUUc -3' miRNA: 3'- gCAAGC-CGCGC----------UAC-CUACGCUuCAGc -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 19127 | 0.66 | 0.775745 |
Target: 5'- --cUCGGCGCGAUcgugGGcgGCGcGGUg- -3' miRNA: 3'- gcaAGCCGCGCUA----CCuaCGCuUCAgc -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 25745 | 0.67 | 0.734146 |
Target: 5'- cCGgUCGGCGUGAaGGucgguUGCGGgacguuGGUCGu -3' miRNA: 3'- -GCaAGCCGCGCUaCCu----ACGCU------UCAGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 37801 | 0.67 | 0.723456 |
Target: 5'- gCGaagCGGCGCGAUGcGAaGCGc-GUCGc -3' miRNA: 3'- -GCaa-GCCGCGCUAC-CUaCGCuuCAGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 38031 | 0.67 | 0.722381 |
Target: 5'- cCGUUCgauaccuGGCGCcccGAccUGGAUGCGAAG-Ca -3' miRNA: 3'- -GCAAG-------CCGCG---CU--ACCUACGCUUCaGc -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 45076 | 0.69 | 0.624458 |
Target: 5'- gGUUCGGCGCGcUGucgaacGAgGCGAAGaUCGc -3' miRNA: 3'- gCAAGCCGCGCuAC------CUaCGCUUC-AGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 41585 | 0.69 | 0.602262 |
Target: 5'- uGggCGGCGCGccGUGGcgGCGAug-CGg -3' miRNA: 3'- gCaaGCCGCGC--UACCuaCGCUucaGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 20974 | 0.7 | 0.52585 |
Target: 5'- gCGcgCGGCGCGGgcgagUGGGUGCGuu-UCGa -3' miRNA: 3'- -GCaaGCCGCGCU-----ACCUACGCuucAGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 19835 | 0.71 | 0.515203 |
Target: 5'- --aUCGGCGCGAUGGuacgAUGCGcauGuUCGg -3' miRNA: 3'- gcaAGCCGCGCUACC----UACGCuu-C-AGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 32278 | 0.72 | 0.433672 |
Target: 5'- cCGgaUGGCGCGGgucGGcgGCGAuGUCGa -3' miRNA: 3'- -GCaaGCCGCGCUa--CCuaCGCUuCAGC- -5' |
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17017 | 3' | -53.7 | NC_004333.2 | + | 38953 | 1.1 | 0.001161 |
Target: 5'- cCGUUCGGCGCGAUGGAUGCGAAGUCGg -3' miRNA: 3'- -GCAAGCCGCGCUACCUACGCUUCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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