Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17028 | 3' | -68.3 | NC_004333.2 | + | 44969 | 0.69 | 0.111515 |
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Target: 5'- gGCGGGCGGcGCGCcg-GGCcgGCUCaCCGg -3' miRNA: 3'- gCGCCCGCC-CGCGaugCCG--CGGG-GGC- -5' |
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| 17028 | 3' | -68.3 | NC_004333.2 | + | 26594 | 0.71 | 0.075201 |
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Target: 5'- uGCGGGCGGGCGUcGCGuaaGCGUUgCUGu -3' miRNA: 3'- gCGCCCGCCCGCGaUGC---CGCGGgGGC- -5' |
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| 17028 | 3' | -68.3 | NC_004333.2 | + | 9297 | 0.72 | 0.05761 |
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Target: 5'- aGCGGcGCGGGUGCgcggacggGCGGCGUUcguauggcgCCCGg -3' miRNA: 3'- gCGCC-CGCCCGCGa-------UGCCGCGG---------GGGC- -5' |
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| 17028 | 3' | -68.3 | NC_004333.2 | + | 34313 | 0.77 | 0.025538 |
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Target: 5'- gCGCGGcGCGGcGCGCUcaaugcgcucgacGCGGCGCCCgUCGu -3' miRNA: 3'- -GCGCC-CGCC-CGCGA-------------UGCCGCGGG-GGC- -5' |
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| 17028 | 3' | -68.3 | NC_004333.2 | + | 35809 | 1.08 | 0.000077 |
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Target: 5'- gCGCGGGCGGGCGCUACGGCGCCCCCGa -3' miRNA: 3'- -GCGCCCGCCCGCGAUGCCGCGGGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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