Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17028 | 5' | -51.9 | NC_004333.2 | + | 19863 | 0.7 | 0.648117 |
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Target: 5'- uGGCGcGAGUGCGAcCGaUUAcGCCCGCGc -3' miRNA: 3'- -UUGCaCUUACGCUuGC-AGU-UGGGCGC- -5' |
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| 17028 | 5' | -51.9 | NC_004333.2 | + | 17250 | 0.7 | 0.648117 |
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Target: 5'- gGACGUGAucgucGCGAugaaggcaaGCGUCGACgCGCu -3' miRNA: 3'- -UUGCACUua---CGCU---------UGCAGUUGgGCGc -5' |
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| 17028 | 5' | -51.9 | NC_004333.2 | + | 38743 | 0.7 | 0.636733 |
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Target: 5'- gGACGgcgaaccgGAcgGCGuGCG-CAGCCCGCa -3' miRNA: 3'- -UUGCa-------CUuaCGCuUGCaGUUGGGCGc -5' |
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| 17028 | 5' | -51.9 | NC_004333.2 | + | 46150 | 0.71 | 0.557502 |
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Target: 5'- cGACGUGAucgAUGCGGccgugACGUCGggcaucAUCCGCGc -3' miRNA: 3'- -UUGCACU---UACGCU-----UGCAGU------UGGGCGC- -5' |
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| 17028 | 5' | -51.9 | NC_004333.2 | + | 2836 | 0.72 | 0.502617 |
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Target: 5'- cGACGUGAAUGCGGcuuCGaUCAGCgUUGCGg -3' miRNA: 3'- -UUGCACUUACGCUu--GC-AGUUG-GGCGC- -5' |
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| 17028 | 5' | -51.9 | NC_004333.2 | + | 35844 | 1.06 | 0.002908 |
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Target: 5'- gAACGUGAAUGCGAACGUCAACCCGCGc -3' miRNA: 3'- -UUGCACUUACGCUUGCAGUUGGGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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