Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17033 | 3' | -53.9 | NC_004333.2 | + | 17697 | 0.66 | 0.710332 |
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Target: 5'- --gCUCGGgcUCGACAAgguaggcgcAGCCGUGAc -3' miRNA: 3'- gagGAGCCgaAGCUGUU---------UCGGCACUa -5' |
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| 17033 | 3' | -53.9 | NC_004333.2 | + | 20353 | 0.66 | 0.699255 |
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Target: 5'- -aCCgcgacCGGCUUCGugAAcGCCGUcGAa -3' miRNA: 3'- gaGGa----GCCGAAGCugUUuCGGCA-CUa -5' |
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| 17033 | 3' | -53.9 | NC_004333.2 | + | 25836 | 0.67 | 0.676906 |
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Target: 5'- uUCgUCGGCUUCcucguGGCGAAGuuGUGc- -3' miRNA: 3'- gAGgAGCCGAAG-----CUGUUUCggCACua -5' |
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| 17033 | 3' | -53.9 | NC_004333.2 | + | 31212 | 0.68 | 0.586614 |
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Target: 5'- uUUCUCGGCUguaccgacgUCGACAaccauaAAGCCGUa-- -3' miRNA: 3'- gAGGAGCCGA---------AGCUGU------UUCGGCAcua -5' |
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| 17033 | 3' | -53.9 | NC_004333.2 | + | 5605 | 0.7 | 0.467307 |
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Target: 5'- cCUCCU-GGCaaCGACGcggauaAGGCCGUGAUg -3' miRNA: 3'- -GAGGAgCCGaaGCUGU------UUCGGCACUA- -5' |
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| 17033 | 3' | -53.9 | NC_004333.2 | + | 32523 | 1.05 | 0.001939 |
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Target: 5'- gCUCCUCGGCUUCGACAAAGCCGUGAUc -3' miRNA: 3'- -GAGGAGCCGAAGCUGUUUCGGCACUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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