Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17035 | 5' | -50.3 | NC_004333.2 | + | 17850 | 0.66 | 0.886046 |
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Target: 5'- -cGGUGCgGcCGCGCGUAuggcggguGGugUCGCa -3' miRNA: 3'- cuUCACGaCaGCGUGCAU--------CUugAGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 33595 | 0.67 | 0.852515 |
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Target: 5'- cGAAGccGCUGUCcCAUGUcgcAGAuacGCUCACa -3' miRNA: 3'- -CUUCa-CGACAGcGUGCA---UCU---UGAGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 32524 | 0.68 | 0.814876 |
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Target: 5'- gGAAGcguuUGCcgacGUCGCGCGgcGcGCUCGCg -3' miRNA: 3'- -CUUC----ACGa---CAGCGUGCauCuUGAGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 10826 | 0.68 | 0.804903 |
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Target: 5'- ----cGUUG-CGCACGUGGAGCggcCGCa -3' miRNA: 3'- cuucaCGACaGCGUGCAUCUUGa--GUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 26843 | 0.68 | 0.794727 |
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Target: 5'- cGAGUGCUGUCGCGuCGc---GCUCGg -3' miRNA: 3'- cUUCACGACAGCGU-GCaucuUGAGUg -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 48033 | 0.69 | 0.77382 |
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Target: 5'- uGgcGUGUUGggCGCugGUGGuuuguGCUCAUg -3' miRNA: 3'- -CuuCACGACa-GCGugCAUCu----UGAGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 28738 | 0.7 | 0.696209 |
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Target: 5'- gGAGGcGCUGgcaCGCGCGU-GAACgUCGCg -3' miRNA: 3'- -CUUCaCGACa--GCGUGCAuCUUG-AGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 5901 | 0.7 | 0.684738 |
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Target: 5'- ---uUGCggccgGUCGCuuucuCGUAGAACUUACg -3' miRNA: 3'- cuucACGa----CAGCGu----GCAUCUUGAGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 39978 | 0.71 | 0.654682 |
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Target: 5'- uGAGGUGCUGgcguuuuugacuggCGCGuuCGUAGGACugaUCACg -3' miRNA: 3'- -CUUCACGACa-------------GCGU--GCAUCUUG---AGUG- -5' |
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| 17035 | 5' | -50.3 | NC_004333.2 | + | 31535 | 1.1 | 0.002209 |
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Target: 5'- cGAAGUGCUGUCGCACGUAGAACUCACc -3' miRNA: 3'- -CUUCACGACAGCGUGCAUCUUGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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