Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1704 | 5' | -53.2 | NC_001347.2 | + | 76963 | 0.66 | 0.988141 |
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Target: 5'- -uGGaUGCUGGaaGUCuUGCCGgcgccCGCCGUg -3' miRNA: 3'- guCCaAUGACU--UAG-ACGGCa----GCGGCA- -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 108203 | 0.67 | 0.981126 |
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Target: 5'- gCAGGcaGCaugGAGUCgcGCgGUCGCCGUu -3' miRNA: 3'- -GUCCaaUGa--CUUAGa-CGgCAGCGGCA- -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 143700 | 0.67 | 0.981126 |
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Target: 5'- --aGUUGCUGGAgcUCU-CCGUCGCCu- -3' miRNA: 3'- gucCAAUGACUU--AGAcGGCAGCGGca -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 199714 | 0.67 | 0.976627 |
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Target: 5'- gAGGcgcgcccGCUGcGAUCUGCgGUUGCCGa -3' miRNA: 3'- gUCCaa-----UGAC-UUAGACGgCAGCGGCa -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 52491 | 0.68 | 0.965353 |
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Target: 5'- uCGGGUgugGCUGucuGUUUGuCUGUgCGCCGUg -3' miRNA: 3'- -GUCCAa--UGACu--UAGAC-GGCA-GCGGCA- -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 31671 | 0.7 | 0.910391 |
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Target: 5'- uCGGGUcgGCUG-GUCggGCCGUCGCgGa -3' miRNA: 3'- -GUCCAa-UGACuUAGa-CGGCAGCGgCa -5' |
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| 1704 | 5' | -53.2 | NC_001347.2 | + | 93817 | 1.07 | 0.01087 |
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Target: 5'- cCAGGUUACUGAAUCUGCCGUCGCCGUu -3' miRNA: 3'- -GUCCAAUGACUUAGACGGCAGCGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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