Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17045 | 5' | -51.9 | NC_004333.2 | + | 36354 | 0.71 | 0.531528 |
Target: 5'- aGCUGGagCGCGCCGuCGCGcuGAu- -3' miRNA: 3'- gCGACUaaGCGCGGCuGCGUuuCUuu -5' |
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17045 | 5' | -51.9 | NC_004333.2 | + | 19546 | 0.72 | 0.502965 |
Target: 5'- aGCUGAUggGCGCCGGuCGCAcggucggcgcggcacAAGggGa -3' miRNA: 3'- gCGACUAagCGCGGCU-GCGU---------------UUCuuU- -5' |
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17045 | 5' | -51.9 | NC_004333.2 | + | 39236 | 0.72 | 0.46667 |
Target: 5'- aCGC-GAUggacaGCGCCGGCGCGAAcGAAGc -3' miRNA: 3'- -GCGaCUAag---CGCGGCUGCGUUU-CUUU- -5' |
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17045 | 5' | -51.9 | NC_004333.2 | + | 41581 | 0.77 | 0.25006 |
Target: 5'- gCGCUGGgcggCGCGCCGugGCGgcGAu- -3' miRNA: 3'- -GCGACUaa--GCGCGGCugCGUuuCUuu -5' |
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17045 | 5' | -51.9 | NC_004333.2 | + | 26178 | 1.07 | 0.002334 |
Target: 5'- gCGCUGAUUCGCGCCGACGCAAAGAAAa -3' miRNA: 3'- -GCGACUAAGCGCGGCUGCGUUUCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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