Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17047 | 5' | -53.3 | NC_004333.2 | + | 17874 | 0.68 | 0.697744 |
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Target: 5'- uGGUGUCGcagGcGCGACGGCGGccagcUCGCc -3' miRNA: 3'- -CUACAGCa--C-UGCUGCCGCCcaa--AGUG- -5' |
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| 17047 | 5' | -53.3 | NC_004333.2 | + | 25417 | 0.68 | 0.697744 |
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Target: 5'- -cUGcagCGUGcCGGCGGCGGccgUCACg -3' miRNA: 3'- cuACa--GCACuGCUGCCGCCcaaAGUG- -5' |
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| 17047 | 5' | -53.3 | NC_004333.2 | + | 24964 | 0.7 | 0.530953 |
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Target: 5'- cGUGaUCaaGACGGCGGCGGGUacggguaUCACg -3' miRNA: 3'- cUAC-AGcaCUGCUGCCGCCCAa------AGUG- -5' |
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| 17047 | 5' | -53.3 | NC_004333.2 | + | 8294 | 0.73 | 0.380604 |
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Target: 5'- uGAUGUagGUaGGCGGCGcGCGGGgcUCGCg -3' miRNA: 3'- -CUACAg-CA-CUGCUGC-CGCCCaaAGUG- -5' |
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| 17047 | 5' | -53.3 | NC_004333.2 | + | 41594 | 0.74 | 0.345558 |
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Target: 5'- --cGcCGUGGCGGCGauGCGGGcUUUCGCg -3' miRNA: 3'- cuaCaGCACUGCUGC--CGCCC-AAAGUG- -5' |
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| 17047 | 5' | -53.3 | NC_004333.2 | + | 25606 | 1.09 | 0.001409 |
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Target: 5'- cGAUGUCGUGACGACGGCGGGUUUCACc -3' miRNA: 3'- -CUACAGCACUGCUGCCGCCCAAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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