Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 17050 | 5' | -54.4 | NC_004333.2 | + | 23874 | 0.74 | 0.28845 |
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Target: 5'- aCGACGUUcggguucGUGCCGCCGUUCaucgugcggaAGCCGa -3' miRNA: 3'- cGUUGUAGu------CACGGCGGCAAG----------UCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 12400 | 0.74 | 0.28845 |
|
Target: 5'- gGC-GCGcCAGUGUCGCCGccUUCGGCCa -3' miRNA: 3'- -CGuUGUaGUCACGGCGGC--AAGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 20884 | 0.75 | 0.253443 |
|
Target: 5'- cGCAuCAUCGGUuucGCCGgcucguggcgaCCGUUCAGCCa -3' miRNA: 3'- -CGUuGUAGUCA---CGGC-----------GGCAAGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 43322 | 0.76 | 0.222005 |
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Target: 5'- aGCGGCAUCGGcacgGCgCGCgCGUacggUCAGCCGa -3' miRNA: 3'- -CGUUGUAGUCa---CG-GCG-GCA----AGUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 39189 | 0.78 | 0.151224 |
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Target: 5'- cGCcGCGUCGGUcGCCGCCGcgcaCGGCCGc -3' miRNA: 3'- -CGuUGUAGUCA-CGGCGGCaa--GUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 25061 | 1.12 | 0.000645 |
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Target: 5'- cGCAACAUCAGUGCCGCCGUUCAGCCGa -3' miRNA: 3'- -CGUUGUAGUCACGGCGGCAAGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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