Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 17050 | 5' | -54.4 | NC_004333.2 | + | 34814 | 0.66 | 0.694196 |
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Target: 5'- aUAGCAUCgaaGGUGcCCGCCag-CAGUCGa -3' miRNA: 3'- cGUUGUAG---UCAC-GGCGGcaaGUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 26830 | 0.66 | 0.694196 |
|
Target: 5'- uGCAGCGUCguagcgAGUGCUGUCGcgUCGcGCuCGg -3' miRNA: 3'- -CGUUGUAG------UCACGGCGGCa-AGU-CG-GC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 2713 | 0.66 | 0.716104 |
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Target: 5'- gGCGGCA--GGUGgaCGCCGUagUUGGCCGc -3' miRNA: 3'- -CGUUGUagUCACg-GCGGCA--AGUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 10078 | 0.66 | 0.694196 |
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Target: 5'- uGCGAC--CAGaUGCCggguaaGCCGUUCAGCa- -3' miRNA: 3'- -CGUUGuaGUC-ACGG------CGGCAAGUCGgc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 47803 | 0.66 | 0.726936 |
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Target: 5'- gGCAACucuGUCGGccUGCaGCUGUUCAaagcGCCGa -3' miRNA: 3'- -CGUUG---UAGUC--ACGgCGGCAAGU----CGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 11853 | 0.66 | 0.737669 |
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Target: 5'- cGguACAUCGcuuccGU-CUGCCGgUCGGCCGa -3' miRNA: 3'- -CguUGUAGU-----CAcGGCGGCaAGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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