Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17050 | 5' | -54.4 | NC_004333.2 | + | 42650 | 0.66 | 0.705187 |
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Target: 5'- cGCAGCGUCuc--CCGUCGagCAGCCc -3' miRNA: 3'- -CGUUGUAGucacGGCGGCaaGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 2713 | 0.66 | 0.716104 |
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Target: 5'- gGCGGCA--GGUGgaCGCCGUagUUGGCCGc -3' miRNA: 3'- -CGUUGUagUCACg-GCGGCA--AGUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 47803 | 0.66 | 0.726936 |
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Target: 5'- gGCAACucuGUCGGccUGCaGCUGUUCAaagcGCCGa -3' miRNA: 3'- -CGUUG---UAGUC--ACGgCGGCAAGU----CGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 5589 | 0.66 | 0.726936 |
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Target: 5'- uGCGGcCAUCGG-GCaugCGCaCGUUCAGgCCGu -3' miRNA: 3'- -CGUU-GUAGUCaCG---GCG-GCAAGUC-GGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 8560 | 0.66 | 0.726936 |
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Target: 5'- gGCuGgAUC-GUGuuGCCGaaUUCAGCCa -3' miRNA: 3'- -CGuUgUAGuCACggCGGC--AAGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 11853 | 0.66 | 0.737669 |
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Target: 5'- cGguACAUCGcuuccGU-CUGCCGgUCGGCCGa -3' miRNA: 3'- -CguUGUAGU-----CAcGGCGGCaAGUCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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