Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17050 | 5' | -54.4 | NC_004333.2 | + | 43322 | 0.76 | 0.222005 |
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Target: 5'- aGCGGCAUCGGcacgGCgCGCgCGUacggUCAGCCGa -3' miRNA: 3'- -CGUUGUAGUCa---CG-GCG-GCA----AGUCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 6362 | 0.71 | 0.41558 |
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Target: 5'- gGCGcCGUC-GUGCCGUCGggCAGCUu -3' miRNA: 3'- -CGUuGUAGuCACGGCGGCaaGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 3404 | 0.71 | 0.41558 |
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Target: 5'- cGCGACcUCGGUaacggccaagauGCCGCCG-UCGGUCa -3' miRNA: 3'- -CGUUGuAGUCA------------CGGCGGCaAGUCGGc -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 36433 | 0.7 | 0.454827 |
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Target: 5'- -gAACGUCGGUGCCGgUCGgcu-GCCGg -3' miRNA: 3'- cgUUGUAGUCACGGC-GGCaaguCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 37068 | 0.69 | 0.517201 |
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Target: 5'- gGCGAUuUC-GUGCCGCUGUUC-GUCGc -3' miRNA: 3'- -CGUUGuAGuCACGGCGGCAAGuCGGC- -5' |
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| 17050 | 5' | -54.4 | NC_004333.2 | + | 47803 | 0.66 | 0.726936 |
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Target: 5'- gGCAACucuGUCGGccUGCaGCUGUUCAaagcGCCGa -3' miRNA: 3'- -CGUUG---UAGUC--ACGgCGGCAAGU----CGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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