miRNA: 3'- aCGg----GCGUCCGG-----------CACCGG--CGGCGGUa -5'
miRNA: 3'- aCGG-GcGUC-CGGCACCGG---CGGCGGUa -5'
miRNA: 3'- aCG--GGCGuCCGGcacCGGCGGCGGUa -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1706 | 3' | -67.3 | NC_001347.2 | + | 200317 | 0.69 | 0.334256 |
|
Target: 5'- cGCaucaaCGCAGGCCagguguugcccGUGGUCugGCCGCCGg -3' miRNA: 3'- aCGg----GCGUCCGG-----------CACCGG--CGGCGGUa -5' |
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| 1706 | 3' | -67.3 | NC_001347.2 | + | 208715 | 0.66 | 0.457981 |
|
Target: 5'- gGCCaC-CAGcGCCGUGGUCucgGUCGCCAg -3' miRNA: 3'- aCGG-GcGUC-CGGCACCGG---CGGCGGUa -5' |
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| 1706 | 3' | -67.3 | NC_001347.2 | + | 222751 | 0.67 | 0.416649 |
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Target: 5'- cGCugCCGC-GGCCauuGCCGCCGCCGc -3' miRNA: 3'- aCG--GGCGuCCGGcacCGGCGGCGGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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