Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1706 | 5' | -50.9 | NC_001347.2 | + | 108293 | 0.69 | 0.984628 |
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Target: 5'- ---aGUCGCggcgauacGCCGGUgcUGCCGCAcGAGa -3' miRNA: 3'- ccuaUAGCG--------UGGCUA--ACGGCGUaCUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 168115 | 0.7 | 0.976089 |
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Target: 5'- cGGUGUCGCGCCGAaacaacacGUaggGCAUGAGc -3' miRNA: 3'- cCUAUAGCGUGGCUaa------CGg--CGUACUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 149053 | 0.7 | 0.967712 |
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Target: 5'- uGAguacagCGaCACCGAUagGCUGCGUGAGg -3' miRNA: 3'- cCUaua---GC-GUGGCUAa-CGGCGUACUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 191148 | 0.73 | 0.914095 |
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Target: 5'- ---cGUCGCGCCa--UGCCGCGUGAc -3' miRNA: 3'- ccuaUAGCGUGGcuaACGGCGUACUc -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 87072 | 0.76 | 0.791789 |
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Target: 5'- -----cCGCGCCGGUccaacaGCCGCAUGAGg -3' miRNA: 3'- ccuauaGCGUGGCUAa-----CGGCGUACUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 114182 | 0.76 | 0.782583 |
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Target: 5'- aGAUGUCGCGCgGAcaGCCGC-UGAGc -3' miRNA: 3'- cCUAUAGCGUGgCUaaCGGCGuACUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 222441 | 0.77 | 0.763769 |
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Target: 5'- ---cGUCGagGCCGAUcgacUGCCGCAUGAGa -3' miRNA: 3'- ccuaUAGCg-UGGCUA----ACGGCGUACUC- -5' |
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| 1706 | 5' | -50.9 | NC_001347.2 | + | 92293 | 1.12 | 0.008614 |
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Target: 5'- gGGAUAUCGCACCGAUUGCCGCAUGAGg -3' miRNA: 3'- -CCUAUAGCGUGGCUAACGGCGUACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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