Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17065 | 3' | -54.2 | NC_004333.2 | + | 11295 | 0.66 | 0.712211 |
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Target: 5'- gCGCUguugaaCUGCGCCGUCGccuuguugcacuGGUCGCg- -3' miRNA: 3'- gGUGAa-----GGCGUGGCAGCu-----------UCAGCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 6869 | 0.66 | 0.725191 |
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Target: 5'- aUCGCgcgCCGgGCgagcucggccuCGUCGAAGUCGCc- -3' miRNA: 3'- -GGUGaa-GGCgUG-----------GCAGCUUCAGCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 21776 | 0.66 | 0.735903 |
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Target: 5'- cCCACuUUCCGCGCUGcagCcAAG-CGCUAu -3' miRNA: 3'- -GGUG-AAGGCGUGGCa--GcUUCaGCGAU- -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 20550 | 0.66 | 0.764257 |
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Target: 5'- aCAUggCCGCGCCGcUGAucgucggcgcgucgGGUCGCg- -3' miRNA: 3'- gGUGaaGGCGUGGCaGCU--------------UCAGCGau -5' |
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| 17065 | 3' | -54.2 | NC_004333.2 | + | 4062 | 0.66 | 0.767349 |
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Target: 5'- aCCACcagccgCCGCGaaaCGaugCGAAGUCGCc- -3' miRNA: 3'- -GGUGaa----GGCGUg--GCa--GCUUCAGCGau -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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